左归丸和右归丸对自身免疫性脑脊髓炎大鼠脑组织中凋亡蛋白Fas、Bax表达的影响

目的：观察左、右归丸对实验性自身免疫性脑脊髓炎（experimental autoimmune encephalomyelitis,EAE）大鼠脑组织中凋亡蛋白Fas、Bax表达的影响。方法：用髓鞘碱性蛋白（myelin basic protein68-86,MBP68-86）免疫Lewis大鼠建立EAE模型。观察各组大鼠体重体温变化,饮食和饮水变化,神经功能评分,发病率、死亡率、潜伏期及病程变化。取脑和脊髓,HE染色后进行病理观察;用免疫组化法检测大鼠脑组织中Fas、Bax的表达情况。结果：左归丸组和右归丸组明显减轻病灶区域的炎性细胞浸润,对Fas、Bax的表达均有一定的抑制作用,与激素组类似。结论：左、右归丸均有防治小鼠EAE的作用,其作用机制可能与调节细胞凋亡蛋白Fas、Bax的表达有关。

Effects of Zuogui Pill and Yougui Pill on Expression of Apoptosis Protein Fas and Bax of Brain Tissue in Rat with Experimental Autoimmune Encephalomyelitis

Objective：To observe the effects of Zuogui Pill and Yougui Pill on the expression of apoptosis protein Fas and Bax of brain tissue in rat with experimental autoimmune encephalomyelitis（EAE）.Methods：The Lewis rat were immunized with myelin basic protein（MBP）68-86 to be made EAE model.The animals were measured the body weight,temperature,volume of food and drink as well as graded daily for clinical disability.The rats were observed the incidence,incubation period,mortality rate and the change of disease course.Brain and spinal cord of the rats were harvested and the pathological changes were studied after dying by HE staining.The expression of Fas and Bax in brain and spinal cord of rats were detected by the method by immunohistochemisty.Results：Zuogui Pill and Yougui Pill can relieve the infiltrated inflammatory cells around focal zone,and inhibited the expression of Fas and Bax,similarly the hormone.Conclusion：Zuogui Pill and Yougui Pill have the effects on prevent and treatment the rats with EAE,the mechanism may be related with regulating expression of apoptosis protein Fas and Bax.