双向等位特异PCR技术及其在单核苷酸多态性研究中的应用

目的：建立双向等位特异PCR体系，并将其应用于单核苷酸多态性（SNP）研究工作。方法：通过美国国家生物信息中心（NCBI）的Genbank获取基因序列及其相应位点的SNP信息。利用Primer5．0软件设计引物，并经NCBI的B1ast2．0软件检验其特异性。双向等位基因特异性引物PCR（Bi—PASAPCR），将该技术应用于与张力蛋白在10号染色体上同源缺失的磷酸酶（PTEN）基因SNP的研究。结果：证实该技术具有较高的可靠性和优越性。结论：双向等位特异PCR技术是一种简便、特异性较高、费用低廉和便于推广的SNP检测方法，特别是在群体基因SNP研究中更有优势。

Application of a bidirectional amplification of specific alleles polymerase chain reaction technique in study of SNPs

Objective:To establish a bidirectional amplification of specific alleles polymerase chain reaction(Bi-PASA PCR)technique system and to apply this technique to study of single nucleotide polymorphism(SNP)of genes.Methods: The information of nucleotide sequences and their corresponding SNPs of gene were obtained from genBank of National Central of Biotechnology Information.The primers were designed by Primer 5.0 software and their specificity was tested by NCBI Blast 2.0 software.Results: They successfully designed and established the technique system of Bidirectional amplification of specific alleles Bi-PASA.The method were applied to study SNPs of PTEN(phosphatase and tensin homology deleted on chromosome ten,PTEN).The superiority and stability of Bi-PASA PCR technique on determining SNPs of genes have been tested.Conclusions:The Bi-PASA PCR technique is a new method for determination of SNP with more convenience,more specificity,less cost and easy availably and has much more advantage especially in the study of population gene polymorphism.