二甲磺酸乙烷致间质细胞破坏所引起的成年大鼠睾丸和附睾的组织学变化:形态定量研究

目的:定量研究睾酮分泌剧烈减少所致睾丸和附睾的组织学变化。方法:14只成年 SD 大鼠腹腔内注射二甲磺酸乙烷(EDS,75mg/kg),14只注射生理盐水作为对照。7天后处死各组中的一半动物,过5天后处死另一半。取睾丸和附睾组织块,甲基丙烯酸树脂包埋。用体视学的光学体视框技术估计睾丸内的细胞数,并用其它形态定量研究方法获取另外一些参数。结果:EDS 注射使睾丸内的间质细胞几乎完全消失,但对支持细胞总数没有影响。EDS 注射7天后,生精上皮内可见许多长形精子细胞滞留,附睾管内可见许多圆形精子细胞。EDS 注射12天后,精子细胞和精母细胞的排列明显变疏松,生精细胞之间出现明显的裂隙,裂隙近似放射状朝向生精小管腔;睾丸内的非 B 型精原细胞总数和精母细胞总数与对照组相似,但 B 型精原细胞总数增加59%,而早期(圆形)、中期和晚期(长形)精子细胞总数分别减少37%、72%和52%。结论:EDS 所致精子发生损害主要是(1)精子释放障碍,(2)精子细胞、精母细胞分离并伴有精子形成和成熟分裂障碍。

Histological changes of the testis and epididymis in adult rats as a result of Leydig cell destruction after ethane dimethane sulfonate treatment:a morphometric study

Aim:To quantitatively study the histological changes of the testis and epididymis as a result of a drastic reduction of testosterone secretion.Methods:Fourteen adult Sprague-Dawley rats were injected intraperitoneally with ethane dimethane sulfonate(EDS,75 mg/kg)and the same number of animals were injected with normal saline as a control. At days 7 and 12(after treatment),respectively,half of the animals from each group were killed.The testes and epididymides were removed and tissue blocks embedded in methacrylate resin.The cell number per testis was esti- mated using the stereological optical disector and some other parameters were obtained using other morphometric methods.Results:The EDS treatment resulted in an almost complete elimination of Leydig cells but had no effect on the numbers of Sertoli cells per testis.At day 7 after EDS treatment,many elongated spermatids were retained in the seminiferous epithelium and many round spermatids could be seen in the epididymal ducts.At day 12,a looser arrangement of spermatids and spermatocytes became evident,with apparent narrow empty spaces being formed between germ cells in an approximately radial direction towards the tubule lumen;the numbers(per testis)of non- type B spermatogonia and spermatocytes were similar to controls,whereas that of type B spermatogonia increased by 59%,and that of early round,elongating and late elongated spermatids decreased by 37%,72% and 52%,respectively. Conclusion:The primary spermatogenic lesions following EDS administration were(i)spermiation failure and(ii) detachment of spermatids and spermatocytes associated with impairment in spermiogenesis and meiosis.