| 核苷类似物对K562细胞的抗增殖和诱导分化作用 |
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| 引用本文： | 居小萍,王健民,周虹,夏放,袁有忠. 核苷类似物对K562细胞的抗增殖和诱导分化作用[J]. 第二军医大学学报, 2001, 22(12): 1139-1141 |
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| 作者姓名： | 居小萍 王健民 周虹 夏放 袁有忠 |
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| 作者单位： | 第二军医大学长海医院血液科, |
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| 基金项目： | 上海市优秀学科带头人项目，98BR029， |
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| 摘 要： | 目的：探讨核苷类似物阿昔洛韦（ACV），更昔洛韦（GCV）对人白血病细胞的抗增殖和诱导分化作用。方法：将ACV、GCV分别加入K562细胞培养4d，测定其活细胞数目，克隆形成率，联苯胺染色阳性率，观察光镜形态，组织化学染色，并进行流式细胞分析，端粒酶检测。结果：在ACV，GCV的作用下，K562细胞的增受抑，生长分数降低，并且向具有合成血红蛋白能力的细胞分化，结论：核苷类似物ACV，GCV对K562细胞具有抑制增殖和诱导分化作用，该结果为慢性粒细胞白血病的治疗提示了新的途径。
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| 关 键 词： | 阿昔洛韦 更昔洛韦 慢性髓样白血病 K562细胞 细胞增殖 诱导分化 |
| 文章编号： | 0258-879X(2001)12-1139-03 |
| 修稿时间： | 2001-04-24 |
Nucleoside analog inhibiting proliferation and inducing differentiation of K562 cells |
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| JU Xiao Ping,WANG Jian Min,ZHOU Hong,XIA Fang,YUAN You Zhong. Nucleoside analog inhibiting proliferation and inducing differentiation of K562 cells[J]. Former Academic Journal of Second Military Medical University, 2001, 22(12): 1139-1141 |
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| Authors: | JU Xiao Ping WANG Jian Min ZHOU Hong XIA Fang YUAN You Zhong |
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| Abstract: | Objective: To explore whether nucleoside analog acycolovir(ACV) and gancyclovir(GCV) can inhibit the proliferation and induce the erythro differentiation of a K562 human myeloid leukemia cell line. Methods: K562 cells were cultured with ACV,GCV for 4 d to detect cellular changes. Results: When K562 cells grew in a medium containing ACV,GCV,the cellular growth and division were gradually suppressed, growth fracture decreased and further differentiation towards the cell producing hemoglobins was found. Conclusion: Nucleoside analog ACV and GCV can inhibit proliferation and induce erythro differentiation of K562 cells. |
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| Keywords: | acycolovir ganciclovir leukemia myeloid chronic |
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