大承气汤对盲肠结扎并穿孔引发脓毒症大鼠的免疫调节

目的：探讨大承气汤对盲肠结扎并穿孔引发脓毒症的免疫调节。方法：大鼠随机分为3组（n=26）假手术组、盲肠结扎并穿孔组、大承气汤组。外周血行中性粒细胞吞噬功能测定及白细胞移行抑制实验,直接放射免疫分析方法及酶联免疫吸附法分别测定血清皮质醇及肿瘤坏死因子-α。结果：大承气汤可提高外周血中性粒细胞的吞噬率及改善胸腺指数、白细胞移行抑制指数;减少肿瘤坏死因子-α过度释放,调节血浆皮质醇水平。结论：大承气汤对盲肠结扎并穿孔引发脓毒症有免疫调节作用。

Immunologic Therapy of Dachengqi Decoction on Sepsis Rats Induced by Cecal Ligation and Puncture

Objective： To study the immunologic therapy of Dachengqi decoction on sepsis rats induced by cecal ligation and puncture. Method： Rats were divided into 3 groups, the normal control group,the CLP model group , the CLP ＋ Dachengqi decoction group; Phagocytosis of PMN,thymus index and MII were detected, The serum level of tumor necrosis factor - α and cortisol were detected by means of ELISA and RIA method. Result： Pretreatment with Dachengqi decoction significantly lowed the excessive content of plasma TNF -α, balanced the level of the cortisol , improved the immune function of organ. Conclusion： Dachengqi decoction have immunologic therapy on sepsis agansist CLP.