凋亡调节蛋白FasL，Fas和P53在乳腺癌的表达及其意义

目的 观察人乳腺癌组织、癌旁相对正常乳腺组织中凋亡调节蛋白FasL，Fas、P53的表达，探讨FasL，Fas及P53与乳腺癌发生发展的关系。方法 收集手术切除的人乳腺癌组织和癌周相对正常乳腺组织，用免疫组织化学方法对21例乳腺癌标本进行检测。结果 乳腺癌组织中Fas蛋白表达的阳性率明显低于癌周正常乳腺组织（P〈0．01），有淋巴结转移者明显低于无淋巴结转移者（P〈0．05）。乳腺癌组织中FasL、P53蛋白表达的阳性率均明显高于癌周正常乳腺组织（P〈0．01），有淋巴结转移者明显高于无淋巴结转移者（P〈0．05）。结论 乳腺癌组织中Fas／FasL表达异常使肿瘤逃脱机体自身免疫攻击，促使肿瘤的发生发展，对预测乳腺癌的预后有重要参考价值。突变型P53在乳腺癌中过表达，可能抑制肿瘤凋亡的发生，促进肿瘤生长。

Expression and Significance of fas, FasL and P53 in Breast Cancer of Human

Objectlve To investigate the expressions of Fas,FasL and P53 in breast cancer and paracancerous tissues of human to explore the relationship of tumor gene and apoptosis regulating protein with developing of breastcancer.Methods The human breast cancer tissues and relative normal breast tissues were collected from the samples of 21 patients with breast cancer and analyzed by immunocytochemistry.Results The positive rate of Fas expression in breast cancer was significantly lower than that in relative normal breast tissues(P<0.01)with much lower in lymphatic metastasis than in nonmetastasis.The positive rates of FasL and P53 expressions in breast cancer were significantly higher than those in relative normal breast tissues(P<0.01)with much lower in lymphatic metastasis than in nonmetastasis.Conclusion The abnormal expressions of Fas/FasL in breast cancer suggest that they are valuable in predicting the clinical prognosis of breast cancer,and the overexpression of P53 gene in breast cancer was closely associated with the tumor development.