大鼠脊髓损伤后神经丝蛋白及胶质纤维酸性蛋白表达的变化及意义

[目的]研究脊髓损伤后神经元中神经丝蛋白(NF200)及星形胶质细胞胶质纤维酸性蛋白(GFAP)表达的变化及其关系,探讨不同细胞在再生修复中的作用。[方法]SD大鼠30只,分为正常、伤后1 d,3 d,7 d,14 d,21 d6组,每组5只,用免疫组化染色及图象分析方法观察神经元NF200及星形胶质细胞GFAP的表达,并对二者进行相关性分析。[结果]脊髓损伤后NF200及GFAP的表达均呈进行性增高,二者有显著的相关性;GFAP表达的增高早于NF200的增高,而前角神经元NF200的表达早于后角神经元。[结论]脊髓损伤后,星形胶质细胞可能是刺激神经元功能活跃,促进神经纤维再生的一个重要因素,不同类型神经元对损伤的反应存在着差异。

The Changes of NF200 and GFAP Expression and Their Significance After Spinal Cord Injury in Rats

[Objective]To study the changes of NF200 in neurons and GFAP in astrocytes and their interrelations after spinal cord injury (SCI).[Methods] Thirty SD rats were divided randomly into six groups, the group of control, 1, 3, 7, 14 and 21 day after SCI. There were five rats in each group. Im-munohistochemical technique and image analysis were used to examine the expressions of NF200 in neurons and GFAP in astrocytes, and their correlation was also analysed. [Results] Both NF200 and the GFAP were increased significantly after SCI. The increase of GFAP was earlier than that of NF200, and the neurons in anterior horn expressed NF200 earlier than that in posterior horn. There was a significant positive relationship between the levels of NF200 and GFAP. [Conclusion] The astrocytes probably play an important role in stimulating and promoting the never regeneration, and the reactions of different type neurons to the injury were not the same.