脑梗饮对局性灶脑缺血再灌注损伤保护作用的实验研究

目的观察脑梗饮对大鼠局灶性脑缺血再灌注脑组织损伤的保护作用,并进一步探讨其作用机制。方法采用大脑中动脉栓塞法制作大鼠局灶性脑缺血再灌注损伤模型。分别运用免疫组化、TTC染色和Zea Longa 5分制评分法,观察脑梗饮治疗后脑组织中Bcl-2蛋白表达、梗塞体积以及神经行为变化。结果与模型组比较,治疗组神经功能损害症状明显减轻(P<0.05);脑梗塞体积缩小约40%;在各时点Bcl-2表达均高与模型组(P<0.05)。结论脑梗饮通过益气养阴、活血通络等综合调控措施不仅能上调凋亡抑制基因Bcl-2表达,缩小脑梗塞体积,而且能改善神经损伤症状,减轻脑组织结构损伤,对脑缺血再灌注引起损伤有明显保护作用。

Experimental Study of the Protective Effects of Naogengyin on Cerebral Ischemia -reperfusion Injury in Rats

Objective To measure the protective effects of Naogengyin on cerebral ischemia-reperfusion injury in rat and its mechanisms.Methods The model of focal reperfusion injury after cerebral ischemia in rats was made by middle cerebral artery occlusion.The effect of Naogengyin on expression of Bcl-2 proin,cerebral infarct volume and behavioral change in rats brain tissue were observed by immunohistochemical method,TTC staining and Zea Longa method respectively.Results Compared with the control group,neurological grades in Naogengyin group were lower(P<0.05).The volume of cerebral infarction decreased 40% in treated groups.Bcl-2 expression of the treated groups increased more than that of the control groups in each hour point.There was great difference between the control groups and treated groups in expression of Bcl-2 protein(P<0.05).Conclusion Naogengyin has great cerebral protective effects against ischemia-reperfusion injury by increasing restrain mechanisms of the apoptosis of neuronal cell and expression of Bcl-2 protein,reducing the volume of cerebral infarction and damage to brain tissue.