儿童激素耐药型与激素敏感型肾病综合征尿蛋白组学的初步研究

目的：筛选和鉴定激素耐药型（SRNS）与激素敏感型原发性肾病综合征（SSNS）尿液中差异表达的蛋白质，寻找可能与肾病综合征激素耐药和激素敏感相关的蛋白质。方法：SSNS治疗前后病例、SRNS、正常对照各5例，提取尿液全蛋白，采用双向凝胶电泳建立全蛋白凝胶图谱；银染后用Image Master 2D V3.01软件分析差异表达蛋白质点，利用MALDI-TOF-MS分析获得肽质量指纹图谱，通过Mascot软件进入NCBI数据库，鉴定出差异表达蛋白质。结果：SRNS组与SSNS治疗前组尿液2-DE凝胶比较有差异蛋白质点66个，其中仅出现于SRNS组24个、SSNS治疗前组27个；SSNS治疗后组与正常对照组尿液2-DE凝胶比较有差异蛋白质点75个，其中仅出现于SSNS治疗后组11个。对18个差异蛋白质点进行分析，鉴定出α1-抗胰蛋白酶、转铁蛋白、带异类电荷糖蛋白复合体等9类差异表达的蛋白质。结论：成功鉴定了SRNS和SSNS的尿液中存在9类差异表达的蛋白质，部分蛋白可能是SRNS或SSNS的标志物。［中国当代儿科杂志，2009，11（5）：341-345］

Preliminary analysis of urinary proteomics in children with steroid resistant and steroid sensitive nephrotic syndrome

Objective To study and identify the protein markers in the urine of children with steroid-sensitive (SSNS) and steroid-resistant nephrotic syndrome(SRNS).Methods Total urinary proteins were extracted from children with SSNS before and after steroid therapy,SRNS,and healthy children (n=5 in each group). Urinary proteins were separated by immobilized pH gradient based on two-dimensional gel electrophoresis (2-DE). The silver-stained 2-DE gels were scanned with digital Image Scanner and analyzed with Image Mas...