环孢素A对成纤维细胞表达细胞间粘附分子1的影响

目的　观察环孢素A (cyclosporinA ,CSA)对体外培养的人类口腔粘膜成纤维细胞(fibroblasts ,FB)表达细胞间粘附分子 1 (intercellularadhesionmolecule 1 ,ICAM 1 )的影响 ,了解CSA治疗口腔粘膜疾病的可能机制。方法　从健康的人类口腔粘膜中培养出FB ,向培养基中加入不同浓度的CSA ,培养 48h后用细胞酶联免疫法检测FB的ICAM 1表达水平。结果　表示ICAM 1表达水平高低的A值在健康人口腔粘膜FB中为 0 32 4± 0 0 30 ,CSA在 0 0 75～ 0 30 0ng/L的范围内以浓度 效应依赖关系下调FB的ICAM 1表达水平。结论　CSA对FB表达ICAM 1的水平具有下调作用 ,提示CSA可能是通过下调ICAM 1的表达来减缓口腔粘膜的炎症反应 ,从而达到治疗口腔粘膜疾病的效果

Effects of cyclosporin A on the levels of intercellular adhesion molecule-1 expressed by oral fibroblasts

OBJECTIVE: To investigate the effects of cyclosporin A (CSA) on the level of intercellular adhesion molecule-1 (ICAM-1) expressed by oral fibroblasts (FB). METHODS: The fibroblasts were obtained from normal buccal mucosa (NM-FB) and cultured in vitro. Then the levels of ICAM-1 expressed by fibroblasts incubated with or without CSA in the presence of 10% fetal calf serum for 48 hours at 37 degrees C in 5% CO(2) and air were monitored by using cell-based ELISA for ICAM-1. RESULTS: We demonstrated that NM-FB had ICAM-1 expression (A = 0.324 +/- 0.030), and after 48 hours incubated with CSA the ICAM-1 levels expressed by oral buccal mucosa fibroblasts were dose-dependently decreased. CONCLUSIONS: CSA reduces the levels of ICAM-1 expressed by oral mucosal FB, and may be useful in the treatment of some oral mucosal disorders.