| Institution: | 1. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI-CONICET), Av. Belgrano y Pje. Caseros, 4000, Tucumán, Argentina;2. Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Catamarca, Belgrano 300, 4700, Catamarca, Argentina;1. Instituto de Química San Luis (INQUISAL), CONICET, Chacabuco 917, 5700 San Luis, Argentina;2. Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, 5700 San Luis, Argentina;3. Division of Basic Biomedical Sciences Sanford School of Medicine, University of South Dakota, Vermillion, SD, USA;1. Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France;2. Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada;3. Biozone, Department of Chemical Engineering and Applied Chemistry and BioZone, University of Toronto, 200 College Street, Toronto, Ontario, M5S 3E5, Canada;4. Univ Brest, Ifremer, CNRS, Unité Biologie et Ecologie des Ecosystèmes Marins Profonds, F-29280, Plouzané, France;1. Laboratory of Cyanobacterial Systematics and Stress Biology, Department of Botany, Banaras Hindu University, Varanasi, India;2. Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University, Varanasi, India;1. Bugworks Research India Pvt. Ltd., Centre for Cellular and Molecular Platforms, GKVK, Bellary Road, Bengaluru 560065, Karnataka, India;2. The University of Trans-Disciplinary Health Sciences and Technology (TDU), 74/2, Post Attur via Yelahanka, Bengaluru, Karnataka 560064, India;3. Department of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8503, Japan;1. Faculdade de Farmácia e Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil;2. Laboratório de Análises Bioquímicas e Citológicas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil;3. The Centre for Biomimetic and Therapeutic Research, Focas Research Institute, Technological University Dublin, Dublin, Ireland;4. Chemistry Department, Maynooth University, National University of Ireland, Maynooth, Ireland;5. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;1. Regional Research Station, Terai Zone, Uttar Banga Krishi Viswavidyalaya, Coochbehar-736165, India;2. Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India;3. Department of Crop Physiology, University of Agricultural Science, Bangalore 560012, India;4. Adjunct Faculty, School of Biology, Indian Institute of Science and Educational Research, Thiruvananthapuram 695551, India |
| Abstract: | Global industrialization and natural resources extraction have left cocktails of environmental pollutants. Thus, this work focuses on developing a defined actinobacteria consortium able to restore systems co-contaminated with pollutants occurring in Argentinian environments. In this context, five actinobacteria were tested in solid medium to evaluate antagonistic interactions and tolerance against lindane (LIN), Reactive Black B–V (RBV), phenanthrene (Ph) and Cr(VI). The strains showed absence of antagonism, and most of them tolerated the presence of individual pollutants and their mixtures, except Micromonospora sp. A10. Thus, a quadruple consortium constituted by Streptomyces sp. A5, M7, MC1, and Amycolatopsis tucumanensis DSM 45259T, was tested in liquid systems with individual contaminants. The best microbial growth was observed in the presence of RBV and the lowest on Cr(VI). Removals detected were 83.3%, 65.0% and 52.4% for Ph, RBV and LIN, respectively, with absence of Cr(VI) dissipation. Consequently, the consortium performance was tested against the organic mixture, and a microbial growth similar to the biotic control and a LIN removal increase (61.2%) were observed. Moreover, the four actinobacteria of the consortium survived the mixture bioremediation process. These results demonstrate the potential of the defined actinobacteria consortium as a tool to restore environments co-contaminated with organic pollutants. |
