| 人端粒酶逆转录酶基因和EGFP共表达真核表达载体的构建及鉴定 |
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| 引用本文： | 代飞,吴军,易绍萱,贺伟峰,陈希炜,张小容. 人端粒酶逆转录酶基因和EGFP共表达真核表达载体的构建及鉴定[J]. 重庆医学, 2004, 33(8): 1206-1208 |
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| 作者姓名： | 代飞 吴军 易绍萱 贺伟峰 陈希炜 张小容 |
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| 作者单位： | 第三军医大学西南医院烧伤研究所,重庆市器官移植基础研究所,400038;第三军医大学西南医院烧伤研究所,重庆市器官移植基础研究所,400038;第三军医大学西南医院烧伤研究所,重庆市器官移植基础研究所,400038;第三军医大学西南医院烧伤研究所,重庆市器官移植基础研究所,400038;第三军医大学西南医院烧伤研究所,重庆市器官移植基础研究所,400038;第三军医大学西南医院烧伤研究所,重庆市器官移植基础研究所,400038 |
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| 基金项目： | 国家自然科学基金 , 国家重点实验室基金 |
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| 摘 要： |
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| 关 键 词： | hTERT 双基因共表达 pIRES2-EGFP |
| 文章编号： | 1671-8348(2004)08-1206-03 |
Construction and identification of eukaryotic expression vector to co-express hTERT and EGFP |
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| DAI Fei,WU Jun,YI Shao xuan,et al.. Construction and identification of eukaryotic expression vector to co-express hTERT and EGFP[J]. Chongqing Medical Journal, 2004, 33(8): 1206-1208 |
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| Authors: | DAI Fei WU Jun YI Shao xuan et al. |
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| Abstract: | Objective To construct and identify a recombinant eukaryotic expression plasmid to express both hTERT and EGFP.Methods pGRN145 plasmid was amplified and cut by restriction endonuclease,then the hTERT sequence was collected and recombined with eukaryotic expression vector pIRES2 EGFP by using gene recombination technigue.At last,the recombinant plasmid was identified and transduced into NIH3T3 cells using Nucleofector TM technique.Results The recombinant dicistronic eukaryotic expression plasmid of hTERT and EGFP genes has been constructed and transduced into NIH3T3 cells succesfully.Conclusion The recombinant plasmid hTERT pIRES2 EGFP can be transduced into cells and expressed EGFP successfully.This result a convenient way to detect whether hTERT gene has been transduced into cells,as well as screening positive cells. |
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| Keywords: | hTERT dicistronic pIRES2 EGFP gane recombination technique |
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