| 抗人肺癌单克隆抗体(3D3)的研制及其特性鉴定 |
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| 引用本文： | 颜江华,杨善民,孙亚敏,陈瑞川,郑耘,陈山厦,陈锦辉. 抗人肺癌单克隆抗体(3D3)的研制及其特性鉴定[J]. 细胞与分子免疫学杂志, 1992, 0(3) |
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| 作者姓名： | 颜江华 杨善民 孙亚敏 陈瑞川 郑耘 陈山厦 陈锦辉 |
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| 作者单位： | 厦门大学抗癌研究中心，厦门大学抗癌研究中心，厦门大学抗癌研究中心，厦门大学抗癌研究中心，厦门大学抗癌研究中心，厦门大学抗癌研究中心，厦门市第一医院病理科 厦门 361005，厦门 361005，厦门 361005，厦门 361005，厦门 361005，厦门 361005，厦门 361000 |
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| 摘 要： | 本文用人肺腺癌组织免疫的BALB/c小鼠脾细胞与小鼠骨髓瘤Sp2/0细胞融合,获得1株单克隆抗体(McAb 3D3)。Ig类测定为IgG_1型。杂交瘤培养上清液和腹水的效价分别为1∶10~3～10~4和1∶10~6。采用间接免疫荧光法(IFA)观察到McAb3D3主要与肺腺癌细胞株A549和L342反应,与其它组织肿瘤细胞株没有反应。ABC免疫酶染色试验显示McAb3D3主要与肺腺癌组织反应,与正常肺组织没有反应。
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| 关 键 词： | 肺肿瘤 抗体 单克隆 |
Preparation and Characterization of Monoclonal Antibody (McAb D) against Human Lung Cancer |
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| Yan Jianghua,Yang Shanmin,Sun Yaping,Chen Ruichuan,Zheng Yun. Chen Xiashan. Preparation and Characterization of Monoclonal Antibody (McAb D) against Human Lung Cancer[J]. Chinese journal of cellular and molecular immunology, 1992, 0(3) |
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| Authors: | Yan Jianghua Yang Shanmin Sun Yaping Chen Ruichuan Zheng Yun. Chen Xiashan |
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| Abstract: | Monoclonal antibody (McAb3D3) was prepared by fusing mice myeloma cells SP2/0 with spleen cells from BALB/c mice immunized with membrane antigens from lung adenocarcinoma. The McAb was identified as IgG and its liter in hybridoma super-natants and ascites was shown by ELISA to be 1:10-10 and 1:10, respectively. Indirect immunofluorescence assay (IFA) showed that McAb3D3 reacted mainly with human lung cancer cells, though it did not react with normal human cells. ABC immunoper-oxidase stain test demonstrated that McAb 3D3 reacted with pulmonary adenocarcinoma but not with normal human tissues |
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| Keywords: | human lung cancer antibody monoclonal |
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