绞股蓝对高脂饵料饲养兔动脉粥样硬化程度和睾酮水平影响研究

目的观察绞股蓝对兔食饵性动脉粥样硬化(As)形成过程中睾酮水平的影响。方法40只月份相似、成年雄性日本大耳白兔随机分为A、B、C、E组,分别喂饲高脂饲料(标准饵料92·5%+2%胆固醇+2%蛋黄+3·5%猪油)+绞股蓝5g/kg、高脂饲料+辛伐他汀5mg/kg、高脂饵料、标准饵料。实验前和实验后6、9周耳中动脉采血检测血脂:甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)和睾酮。观察主动脉病理学形态。结果①血脂:A、B、C组饲养后TG、TC、LDL-C、HDL-C明显升高(P<0·05～0·01),A组和B组TG、TC、LDL-C低于C组(P<0·05～0·01),A组和B组TG、TC、LDL-C低于C组(P<0·05～0·01),A组HDL-C优于C组(P<0·01);②睾酮变化:实验前和实验后6、9周A、B、C、E组组内和组间睾酮差异均无统计学意义(P<0·05);③病理形态学改变:E组血管内膜光滑,无斑块;C组大量灰白色脂样物凸出并连成片状,以主动脉近段明显;A组和B组斑块明显减少,以主动脉近段和血管分支处斑点状斑块明显。A、B、C、E组的斑块/内膜面积比分别为(31±5)%、(32±6)%、(60±9)%、0,A、B与C组间比较差异有统计学意义(P<0·01),光镜下E组内膜光滑,C组内膜增厚,脂质斑块弥漫,泡沫细胞积聚,斑块底部泡沫细胞发生变性坏死,内弹力层和中弹力板发生变性、断裂和崩解;A、B组动脉病变情况较C组明显减轻。A、B、C、E组的内膜/中膜厚度比分别为(40±16)%、(41±15)%、(70±12)%、0,A、B与C组间比较差异有统计学意义(P<0·01)。结论高脂血症、绞股蓝均不影响兔食饵性As形成过程中睾酮水平,绞股蓝不是通过睾酮途径干预As。

The research of prevention effect of fiveleaf gynostemma herb on the rabbits suffered from atherosclerosis and TESTO caused by high cholesterol feedstuff

Objective To study the effect of Fiveleaf Gynostemma Herb on the rabbits which suffered from atherosclerosis(As) and TESTO caused by high cholesterol feedstuff and to discuss the possible mechanism.Methods Forty rabbits were divided as the Fiveleaf Gynostemma Herb interverntion group(group A),Simvastain intervention group(group B),high blood lipids model group(group C),normal-control group(group E) at random,which were fed high fat food(92.5% standard feedstuff added 2% cholesterol,2% yolk and 3.5% lard) added Fiveleaf Gynostemma Herb(5 g/kg),high fat feedstuff added simvastain(5 mg/kg),high fat feedstuff and standard feedstuff respectively.TG,TC,LDL-C,HDL-C and TESTO were examined before the experiment and at the sixth and nineth week of the experiment.Aortic pathology and the thickness of endarterium of the amimals in each group were observed.Results①Blood lipids:with the feeding time prolonged,TG,TC and LDL-C of group A,group B and group C were growing gradually and had the prominent difference than ex-experiment(P<0.05～0.01).HDL-C of group A and group B had the significant differnece(P<0.01).TG,TC,LDL-C of group A and group B were lower than those in group C(P<0.05～0.01).HDL-C of group A was superior to group C(P<0.01);②Level of TESTO:TESTO of group A,group B,group C and group E had no difference before the experiment and at the sixth and nineth week of the experiment(P>0.05);③Pathomorphological examination:the blood vessel of group E is smooth and had no atherosclerotic plaque;group C has many gray grease and it can be easily seen near the aorta;the atherosclerotic plaque area of group A and group B had distinctively decreased.The rate between atherosclerotic plaque and intima of group A,group B,group C and group E was(31±5)%,(32±6)%,(60±9)% and 0 respectively,the rate between intima and extima of group A,group B,group C and group E was(40±16)%,(41±15)%,(70±12)%、0,aortic intimal atherosclerotic plaque area and arterial intima thickness group A and group B of obviously reduced.Group A,group B and group C had the significant difference(P<0.01).Conclusion Fiveleaf Gynostemma Herb had the similar intervention effect as the simvastain to the As rabbits caused by high cholesterol food and this effect was in relation to the adjusting the metabolism of the blood lipids and inhibitory the inflmmation reaction.Fiveleaf Gynostemma Herb had the difference in adjusting the metabolism of the blood lipids with Simvastain.