aFGF对新生大鼠缺氧缺血后VEGF的表达及脑血管新生的作用

目的探讨外源性酸性成纤维细胞生长因子(aFGF)对新生大鼠缺氧缺血性脑损伤血管新生的作用及可能机制。方法以7日龄SD大鼠制作HIBD的动物模型,应用免疫组织化学染色方法观察各组脑血管内皮细胞生长因子(VEGF)的表达及血管新生情况。结果免疫组化检测VEGF的表达,治疗组VEGF的表达从缺氧后第1天开始增加,到第3天时明显高于对照组(P<0.01),第7天3组之间无统计学差别。治疗组的微血管数目在缺氧缺血后第1天显著高于对照组(P<0.01),第3天还高于对照组(P<0.05),第7天仍高于假手术组(P<0.01)。结论aFGF使缺氧缺血大鼠脑组织VEGF表达增多,可能是促进脑组织新生血管形成的重要因素之一,提示早期应用外源性aFGF对于HIBD新生大鼠有一定的治疗作用。

The vascular endothelial growth factor expression and angiogenic response of exogenous acid fibroblast growth factor in neonatal rats with hypoxic-ischemic brain damage

Objective To investigate the angiogenic effect of exogenous acid fibroblast growth factor (aFGF) in neonatal rats with hypoxic-ischemic brain damage(HIBD).Methods A model of HIBD of seven-day-old Sprague-Dawley rats was established. Immunohistochemical techniques were used to evaluate vascular endothelial growth factor ( VEGF) protein localization and angiogenesis.Results VEGF protein expression,as measured in immunohistochemical techniques,was increased in rat brains in the aFGF trail group at 1st day to 3rd day postoperatively.It was significantly increased in the aFGF trail group at 3rd day (P<0.01 vs control group).There was no statistical difference among three groups at 7th day.Microvessel in the rat brains began to increase significantly at 1st day after operation in the trail group compared with control group (P<0.01),as assessed immunohistochemically,and the increase was maintained for 3 days (P<0.05).Microvessel in the trail group was more than that in pseudo operation group even at 7th day(P<0.01).Conclusion These results demonstrated that aFGF could induce sustained up-regulation of VEGF expression in rat brain,which was correlated with angiogenesis.The early treatment with exogenous aFGF can improve the attenuate learning and memory dysfunction following HIBD.