幽门螺杆菌感染的消化性溃疡患儿细胞因子的检测

目的探讨幽门螺杆菌(HP)感染相关的消化性溃疡细胞因子的免疫学发病机制。方法将76例幽门螺杆菌(HP)感染相关的消化性溃疡患儿作为病例组,同期检测50例健康小儿作为对照组,通过ELISA、双抗体夹心ELISA的方法,检测76例幽门螺杆菌(HP)感染相关的消化性溃疡患儿外周血白细胞介素2(IL-2)、可溶性白细胞介素2受体(sIL-2R)、白细胞介素6和8(IL-6、IL-8)及T淋巴细胞亚群的含量,以评价幽门螺杆菌(HP)感染与消化性溃疡患儿细胞因子的关系。结果病例组IL-2水平显著低于对照组(均P<0.01),而CD8+细胞百分率、sIL-2R、IL-6、IL-8水平显著高于对照组(均P<0.01)。结论 HP感染相关的消化性溃疡患儿细胞免疫功能低下且紊乱,该患儿机体的免疫功能障碍在该病的发生中起一定作用。

Detection of cellular immunity in childhood peptic ulcer disease correlated with helicobacter pylori

Objective To examine cytokines in the immunological pathogenesis of peptic ulcer disease correlated with helicobacter pylori （HP）. Methods 76 cases of Helieobaeter pylori （HP） infection in children with peptic ulcer dis- ease as a case group and the earlier detection of 50 healthy children as control group. The peripheral blood interleukin 2 （IL-2）, soluble interleukin2 receptor （slL-2R）, interleukin 6and 8（IL-6and IL-8） and T lymphocyte subsets were detec- ted with double antibody sandwich ELISA or ELISA. Results IL-2 levels of the patients were significantly lower than that in control group （P〈0. 01）, and the percentage of CDS＋ cells, slL-2R, IL-6, IL-8 levels of the patients were sig- nificantly higher than tahat in control group （P〈0. 01）. Conclusion Cellular immunity function is low and disorder in childhood peptic ulcer disease correlated with HP and this disorders in cellular immunity function have some effect in Its pathogenesis.