Influence of age‐related degeneration on regenerative potential of human nucleus pulposus cells |
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| Authors: | Shu‐Hua Yang Chen‐Chiang Lin Ming‐Hsiao Hu Tiffany Ting‐Fang Shih Yuan‐Hui Sun Feng‐Huei Lin |
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| Affiliation: | 1. Department of Orthopedics, National Taiwan University Hospital Yun‐Lin Branch, Douliou City, Yunlin County, Taiwan;2. Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan;3. Department of Radiology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan;4. Institute of Biomedical Engineering, College of Engineering and College of Medicine, National Taiwan University, No. 1 Ren‐Ai Road, Taipei, Taiwan |
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| Abstract: | Nucleus pulposus (NP) cells, sourced from herniation surgeries, may be used as a cell‐based therapy for intervertebral disc (IVD) degeneration. But, both the regenerative potential of these degenerative adult NP cells and how to stimulate optimum matrix synthesis is not yet clear. The purpose of the current study was to understand the different phenotypic behaviors between degenerative adult NP cells and normal adolescent NP cells. Degenerative adult NP cells produced a significantly higher amount of proteoglycans and collagens than adolescent cells. Insulin‐like growth factor‐1 was the only anabolic cytokine with increased endogenous expression in degenerative adult NP cells. TGF‐β1 treatment of degenerative NP cells promoted matrix synthesis but stimulated too much type I collagen and suppressed type II collagen and aggrecan. Adult degenerative NP cells possess upregulated regenerative potential, but stimulation in addition to TGF‐β1 is needed to enhance matrix productivity and optimize the collagen expression profile. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:379–383, 2010 |
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| Keywords: | nucleus pulposus degeneration regeneration collagens TGF‐β 1 |
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