Morphological and genetic diversity of symbiotic cyanobacteria from cycads |
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| Authors: | Nooruddin Thajuddin Gangatharan Muralitharan Mariappan Sundaramoorthy Rengasamy Ramamoorthy Srinivasan Ramachandran Mohamed Abdulkadar Akbarsha Muthukumaran Gunasekaran Prof. Dr. |
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| Affiliation: | 1. Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli Tamilnadu, India;2. Rice Functional Genomics Group, Temasek Life Sciences Laboratory, 1, Research Link, The National University of Singapore, Singapore;3. Department of Animal Sciences, School of Life Sciences, Bharathidasan University, Tiruchirappalli Tamilnadu, India;4. Department of Biology, Fisk University, Nashville TN, USA |
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| Abstract: | The morphological and genetic diversity of cyanobacteria associated with cycads was examined using PCR amplification techniques and 16S rRNA gene sequence analysis. Eighteen symbiotic cyanobacteria were isolated from different cycad species. One of the symbiotic isolates was a species of Calothrix, a genus not previously reported to form symbioses with Cycadaceae family, and the remainder were Nostoc spp. Axenic cyanobacterial strains were compared by DNA amplification using PCR with either short arbitrary primers or primers specific for the repetitive sequences. Based on fingerprint patterns and phenograms, it was revealed that cyanobacterial symbionts exhibit important genetic diversity among host plants, both within and between cycad populations. A phylogenetic analysis based on 16S rRNA gene sequence analysis revealed that most of the symbiotic cyanobacterial isolates fell into well‐separated clades. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
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| Keywords: | 16S rRNA gene sequence Calothrix Cyanobacteria Cycad Nostoc RAPD STRR‐PCR |
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