Benefits of blood group genotyping in multi‐transfused patients from the south of Brazil |
| |
Authors: | Gláucia Andréia Soares Guelsin Ana Maria Sell Lilian Castilho Viviane Lika Masaki Fabiano Cavalcante Melo Margareth Naomi Hashimoto Tatiana Takahashi Higa Loide Souza Hirle Jeane Eliete Laguila Visentainer |
| |
Affiliation: | 1. Laboratório de Imunogenética, Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Maringá, Brazil;2. Laboratório de Pesquisa em Biologia Molecular de Grupos Sanguíneos, Centro de Hematologia e Hemoterapia, Universidade Estadual de Campinas, Cidade Universitária Zeferino Vaz–Bar?o Geraldo, Campinas, Brazil;3. Hemocentro Regional de Maringá, Universidade Estadual de Maringá, Brazil |
| |
Abstract: | We evaluated the usefulness of blood group genotyping as a supplement to hemagglutination to determine the red blood cell (RBC) antigen profile of polytransfused patients with hematological diseases and renal failure. Seventy‐nine patients were selected. They all received more than three units of blood and eight (10%) had already clinical significant alloantibodies occurring alone or in combination against Rh, K, Fya, and Di antigens. DNA was prepared from blood samples and RHCE*E/e, KEL*01/KEL*02, FY*01/FY*02 and JK*01/JK*02 alleles were determined by using PCR‐RFLP. RHD*/RHD*Ψ and RHCE*C/c were tested using multiplex PCR. Discrepancies for Rh, Kell, Duffy, and Kidd systems were found between the phenotype and genotype‐derived phenotype in 16 of the 38 chronically transfused patients. The genotypes of these patients were confirmed by DNA array analysis (HEA Beadchip?; Bioarray Solutions, Warren, NJ). Genotyping was very important for the determination of the true blood groups of the polytransfused patients, helped in the identification of suspected alloantibodies and in the selection of antigen‐negative RBCs for transfusion. J. Clin. Lab. Anal. 24:311–316, 2010. © 2010 Wiley‐Liss, Inc. |
| |
Keywords: | genotyping assays blood group antigens hemagglutination transfusion |
|
|