Comparison and Evaluation of Several Dermatophagoides Pteronyssinus Allergen Extracts for Skin Prick Test |
| |
| Authors: | Jin-lu Sun Rui-qi Wang Jia Yin Liang-lu wang Hong-yu Zhang Hong Li Yu-xiang Zhi Li-ping Wen Kai Guan Jian-qing Gu Hai-juan He Ying Zhao Wei Zhang Guo-qiang Sun |
| |
| Institution: | 1. School of Nursing, Medical College of Soochow University, Suzhou, China;2. Center for Disease Prevention and Control of Gusu District, Suzhou, China;3. Department of Epidemiology, School of Public Health, Medical College of Soochow University, Suzhou, China;4. Department of Nursing, The First Affiliated Hospital of Soochow University, Suzhou, China;1. Cardiovascular Department, The Second Affiliated Hospital of Nanchang University, Nanchang, China;2. University of Birmingham Centre for Cardiovascular Sciences, City Hospital, Birmingham, United Kingdom |
| |
| Abstract: | To evaluate the significance of several Dermatophagoides pteronyssinus allergen extracts for skin prick test (SPT) in patients allergic to Dermatophagoides pteronyssinus.Methods Two hundred and nineteen patients enrolled in Peking Union Medical College Hospital underwent SPT and serum specific IgE assay to detect the Dermatophagoides pteronyssinus allergen. Three kinds of house dust mite allergen extracts were used for SPT, including the Dermatophagoides pteronyssinus extract prepared by our laboratory (group A), standardized Dermatophagoides pteronyssinus extract (group B), and mixed extracts of Dermatophagoides pteronyssinus and Dermatophagoidesfarinae (group C). Human serum spe-cific IgE result was regarded as the reference standard for diagnosis of Dermatophagoides pteronyssinus allergy.The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic performance of SPT with the extracts of three groups.Results SPT results showed that the median wheal diameter of group A, group B, and group C was 0.43, 0.35, and 0.28 cm, respectively, with significant difference among three groups (P<0.05). The differ-ence was significant between group A and B (P<0.01) as well as group A and C (P<0.01), but not between group B and C (P>0.05). There was no local urticaria or systemic allergic reactions following the procedure of SPT. Local reaction was observed in 5 patients and delayed reaction was in 2 patients of group A. As for group B and C, local reaction occurred in 3 cases and delayed reaction in 2 cases in each group. The area un-der ROC curve of SPT with extract in group A, group B, and group C was 0.765, 0.801, and 0.782, respec-tively. Based on the detection results of serum specific IgE, the sensitivity of SPT in diagnosis of Dermato-phagoides pteronyssinus allergy with extract of group A, group B, and group C was 92.40/0, 87.00/0, and 81.5%,and the specificity was 60.6%, 73.2%, and 74.8%, respectively. |
| |
| Keywords: | Dermatophagoides pteronyssinus allergen skin prick test receiver operatingcharacteristic curve |
| 本文献已被 维普 万方数据 ScienceDirect 等数据库收录！ |
|
