ALDH1和ABCG2在宫颈鳞癌中的表达及其临床意义

目的:检测肿瘤干细胞标志物乙醛脱氢酶1(ALDH1)和三磷酸腺苷结合盒转运蛋白G2(ABCG2)在不同程度宫颈病变组织中的表达情况。方法:应用免疫组化SP法检测76例宫颈鳞癌、52例宫颈高级别鳞状上皮内病变(HSIL)、37例慢性宫颈炎患者的石蜡组织中ALDH1和ABCG2的表达情况,并进行比较分析。结果:1ALDH1和ABCG2在慢性宫颈炎、HSIL、宫颈鳞癌组织中的阳性表达率分别为5.41%(2/37)和8.11%(3/37)、23.08%(12/52)和28.85%(15/52)、53.95%(41/76)和61.84%(47/76),两者在宫颈鳞癌组织中的阳性表达率明显高于HSIL组织、慢性宫颈炎组织,差异均有统计学意义(P0.05)。2ALDH1在宫颈鳞癌中表达水平与肿瘤分化程度、是否存在淋巴结转移有关(P0.05),ABCG2在宫颈鳞癌中表达水平与宫颈癌的临床分期、肿瘤细胞的分化程度及是否有淋巴结转移有关(P0.05)。3宫颈鳞癌组织中ALDH1和ABCG2表达水平水平呈正相关(r=0.535,P0.05)。结论:ALDH1和ABCG2在宫颈鳞癌的表达升高,可能在宫颈鳞癌的发生、发展、转移中起作用。ALDH1和ABCG2在宫颈鳞癌的发展过程中可能起协同作用。

Expression and Clinical Significance of ALDH1 and ABCG2 in Cervical Cancer Cells

Objective:To detect the expression of ALDH1 and ABCG2 in the specimens from different levels of cervical lesions.Methods:Use SP immunohistochemistry to detect the expression of ALDH1 and ABCG2 in 76 cases of cervical squamous cancers,52 cases of HSIL,and 37 cases of chronic cervicitis tissues,and analyze the results.Results:①The positive expression rates of ALDH1 and ABCG2 in chronic cervicitis tissues were 5.41％ (2/37) and 8.11％ (3/37),in HSIL tissues were 23.08％ (12/52) and 28.85％ (15/52),and in cervical cancers tissues were 53.95％ (41/76) and 61.84％ (47/76),respectively.The positive expression rates in cervical cancers tissues were significantly higher than in HSIL and Cc hronic cervicitis tissues,(P ＜ 0.05).②The positive expression of ALDH1 in cervical cancer tissues had correlation with tumor grade and lymphnode metastasis (P ＜0.05),while the positive expression of ABCG2 in cervical cancer tissues had correlation with tumor grade,clinical stage and lymph-node metastasis(P ＜0.05).③The expressions of ALDH1 and ARCG2 in cervical cancer were positive relative(r =0.535,P ＜ 0.05).Conclusions:The increased expressions of ALDH1 and ABCG2 in cervical squamous cancer may play a role in the occurrence,development and metastasis of cervical squamous cell carcinoma.ALDH1 and ABCG2 may have synergistic effect in occurrence of cervical cancer.