DCE-MRI对裸鼠胃癌原位移植瘤的可行性研究

目的 评估动态增强磁共振成像(DCE-MRI)用于裸鼠胃癌原位移植瘤模型研究的可行性.方法 建立裸鼠胃癌原位移植瘤模型,15只采用钆喷酸葡胺注射液为对比剂行DCE-MRI,扫描后取出移植瘤瘤体检测微血管密度(MVD).并对比裸鼠胃癌原位移植瘤模型MVD与正常胃黏膜组织MVD的差异.结果 15只裸鼠胃癌原位移植瘤模型成功行MRI平扫及DCE-MRI,裸鼠胃癌原位移植瘤微血管转运常数(Ktrans)值为(2.11±0.44) min-1,反流速率常数(Kep)值为(4.59±0.93) min-1, 血管外细胞外容积分数(Ve)值为0.46±0.06.裸鼠胃癌原位移植瘤中MVD显著高于正常胃黏膜组织(χ2=16.205,P<0.001).结论 DCE-MRI可以无创定量评估胃恶性肿瘤血管参数.

Application of DCE-MRI in orthotopic transplantation model of gastric cancer in nude mice

Objective To study the feasibility of DCE-MRI application in the orthotopic transplantation model of gastric cancer in nude mice.Methods Orthotopic transplantation model of gastric cancer in nude mice was established, and 15 models underwent DCE-MRI using the contrast agent of gadopentetate.Subsequently, the tumor was dissected in order to detect the MVD.The MVD was compared between orthotopic transplantation tumor model of gastric cancer and normal gastric mucosa.Results Fifteen nude mice with orthotopic transplantation of gastric cancer successfully underwent DCE-MRI examination.As for the cancer, the values of Ktrans, Kep and Ve were (2.11±0.44) min-1, (4.59±0.93) min-1, and 0.46±0.06, respectively.The MVD in gastric cancer tissues was significantly higher than that in normal gastric mucosa (χ2=16.205, P<0.001).Conclusion DCE-MRI can be used for noninvasively quantitative evaluation of vascular parameters of gastric carcinoma.