过表达P57KIP2基因对滋养层细胞生物学行为的影响 |
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引用本文: | 刘广钰, 刘兴会, 许文明, 何国琳. 过表达P57KIP2基因对滋养层细胞生物学行为的影响[J]. 实用妇产科杂志, 2017, 33(8): 626-630 |
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作者姓名: | 刘广钰 刘兴会 许文明 何国琳 |
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作者单位: | 四川大学华西第二医院出生缺陷与相关妇儿疾病教育部重点实验室,四川成都,610041 |
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基金项目: | 国家自然科学基金项目(81471463),四川省科技厅课题(2014JY0158) |
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摘 要: | 目的:探究P57KIP2基因过表达之后对滋养层细胞生物学行为的影响。方法:采用实时荧光定量(q PCR)及Western Blot方法检测人绒毛膜滋养层细胞(HTR8-/SVneo)过表达P57KIP2基因后mRNA和蛋白水平的表达变化;用Transwell方法检测HTR8-/SVneo细胞过表达P57KIP2基因后迁移、侵袭能力生物学行为的变化;CCK8检测HTR8-/SVneo细胞过表达该基因后增殖能力的变化。采用免疫荧光检测HTR8-/SVneo细胞过表达该基因后,抗凋亡蛋白Bcl2的表达变化。结果:滋养层细胞过表达P57KIP2基因后,其mRNA及蛋白水平均明显增高(P0.05);P57KIP2基因过表达后滋养层细胞的迁移、侵袭数量均明显增高,差异有统计学意义(P0.05);滋养层细胞的细胞相对吸光值48小时后明显增高(P0.001);过表达P57KIP2基因后滋养层细胞中抗凋亡蛋白Bcl2表达增高(P0.05)。结论:P57KIP2基因可能会增加滋养层细胞迁移、侵袭以及增殖能力,P57KIP2基因可能参与调控滋养细胞的凋亡过程。
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关 键 词: | 子痫前期 P57KIP2基因 迁移 侵袭 凋亡 |
Overexpression P57KIP2 Gene Affects Human Extravillous Trophoblast Cell Behaviors |
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Abstract: | Objective:To check the effect P57KIP2 overexpression on biological behaviors of trophoblast cell.Methods:After P57KIP2 overexpression,the mRNA and protein expression of P57KIP2 were detected by real-time fluorescence quantitative(qPCR) and Western Blot to detectin human trophoblast cell(HTR8-/SVneo).The change of migration and invasion were test by Transwell method,the changes the proliferation was detected by CCK8;the change of the expression of anti-apoptotic protein Bcl2 was detected by immunofluorescence staining.Results:Overexpresion P57KIP2 significantly increased the relative mRNA and protein expression of P57KIP2 gene(P < 0.05);and the number of imgration and invasion of trophoblast cells were significantly increased(P < 0.05);and the proliferation of trophoblast cells was significantly enhanced after 48 hours (P <0.001);the expression of antiapoptotic protein Bcl2 was increased in human trophoblast cells (P < 0.05).Conclusions:P57KIP2 gene may increase the ability of migration,invasion and proliferation of trophoblast cells.P57KIP2 gene may be involved in the regulation of apoptosis of trophoblast cell. |
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Keywords: | Preeclampsia P57KIP2 Migration Invasion Apoptosis |
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