过表达P57KIP2基因对滋养层细胞生物学行为的影响

目的:探究P57KIP2基因过表达之后对滋养层细胞生物学行为的影响。方法:采用实时荧光定量(q PCR)及Western Blot方法检测人绒毛膜滋养层细胞(HTR8-/SVneo)过表达P57KIP2基因后mRNA和蛋白水平的表达变化;用Transwell方法检测HTR8-/SVneo细胞过表达P57KIP2基因后迁移、侵袭能力生物学行为的变化;CCK8检测HTR8-/SVneo细胞过表达该基因后增殖能力的变化。采用免疫荧光检测HTR8-/SVneo细胞过表达该基因后,抗凋亡蛋白Bcl2的表达变化。结果:滋养层细胞过表达P57KIP2基因后,其mRNA及蛋白水平均明显增高(P0.05);P57KIP2基因过表达后滋养层细胞的迁移、侵袭数量均明显增高,差异有统计学意义(P0.05);滋养层细胞的细胞相对吸光值48小时后明显增高(P0.001);过表达P57KIP2基因后滋养层细胞中抗凋亡蛋白Bcl2表达增高(P0.05)。结论:P57KIP2基因可能会增加滋养层细胞迁移、侵袭以及增殖能力,P57KIP2基因可能参与调控滋养细胞的凋亡过程。

Overexpression P57KIP2 Gene Affects Human Extravillous Trophoblast Cell Behaviors

Objective:To check the effect P57KIP2 overexpression on biological behaviors of trophoblast cell.Methods:After P57KIP2 overexpression,the mRNA and protein expression of P57KIP2 were detected by real-time fluorescence quantitative(qPCR) and Western Blot to detectin human trophoblast cell(HTR8-/SVneo).The change of migration and invasion were test by Transwell method,the changes the proliferation was detected by CCK8;the change of the expression of anti-apoptotic protein Bcl2 was detected by immunofluorescence staining.Results:Overexpresion P57KIP2 significantly increased the relative mRNA and protein expression of P57KIP2 gene(P ＜ 0.05);and the number of imgration and invasion of trophoblast cells were significantly increased(P ＜ 0.05);and the proliferation of trophoblast cells was significantly enhanced after 48 hours (P ＜0.001);the expression of antiapoptotic protein Bcl2 was increased in human trophoblast cells (P ＜ 0.05).Conclusions:P57KIP2 gene may increase the ability of migration,invasion and proliferation of trophoblast cells.P57KIP2 gene may be involved in the regulation of apoptosis of trophoblast cell.