肺癌细胞钙黏蛋白E及连环蛋白的差异表达

目的测定不同转移能力肺癌细胞上皮型钙黏蛋白（E-cad），α,β-连环蛋白（α-catenin、β-catenin）的差异表达，探讨它们在肺癌侵袭、转移机制中的作用。方法将高侵袭的肺腺癌细胞（A549）、高转移的肺癌细胞（95-D）、低转移的肺腺癌细胞（SPC-A-1）作常规细胞培养，采用Westen-blot免疫印迹法和逆转录-聚合酶链反应分别从蛋白质和mRNA水平上检测上皮型E-cad及α、β-catenin的表达情况。结果A549与95-D细胞株的E-cad及α、β-catenin表达量较低，95-D表达最弱，二者之间差异无统计学意义（P〉0．05）；SPC—A-1细胞株除E-cad弱表达外，α、β-catenin与95-D和A549比较呈明显强表达，差异有统计学意义（P〈0．05）。结论高转移能力的肺癌细胞E-cad和α、β-catenin表达低，低转移的肺癌细胞E-cad和α、β-catenin表达高，表明E-cad及cateinis在肺癌细胞侵袭和转移中起重要作用。

Study on the differential expression of E-cadherin and catenins in lung cancer cell

Objective To observe the differential expression levels of E-cadherin and α,β-catenins in three lung cancer cell lines with differentially metastatic abilities,in order to study their mechanism of adhesion and metastasis in lung cancer.Methods The three lung cancer cell lines:A549(highly invasive human lung adenocarcinoma cell),95-D(highly metastatic human lung carcinoma cell),and SPC-A-1(lowly metastatic human lung adenocarcinoma cell)were cultured conventionally in vitro.The protein and mRNA expression level of E-cadherin and catenins were observed by western blot analysis and RT-PCR respectively1].Results The total protein and mRNA expression levels of E-cadherin and catenin in A549 and 95-D cells were lower,while the lowest was in 95-D.But there were no significant difference (P＞0.05).In SPC-A-1 cell line,the expression level of E-cadherin was low.But the expression levels of catenins were significantly higher than those of the other groups(P＜0.05).Conclusion In highly metastatic cells 95-D and A549,the expression levels of E-cadherin and catenins were lower,which lead to invasion and metastasis .In low metastatic SPC-A-1,the expression level was high.The study indicated that E-cadherin and catenins may play an important role in the mechanisms of invasion and metastasis of lung cancer.