Effects of the combination of low-level laser irradiation and recombinant human bone morphogenetic protein-2 in bone repair |
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| Authors: | Anderson Paim Rosa Luiz Gustavo de Sousa Simone Cecilio Hallak Regalo Jo?o Paulo Mardegan Issa Ana Paula Amorim Barbosa Dimitrius Leonardo Pitol Richard Honorato de Oliveira Paulo Batista de Vasconcelos Fernando José Dias Daniela Thomazatti Chimello Selma Siéssere |
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| Affiliation: | Department of Morphology, Stomatology and Physiology, School of Dentistry at Ribeir?o Preto, University of S?o Paulo, Avenida do Café, s/n - Bairro - Monte Alegre, 14040-904, Ribeir?o Preto, S?o Paulo, Brazil. |
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| Abstract: | Low-level laser irradiation (LLLI) and recombinant human bone morphogenetic protein type 2 (rhBMP-2) have been used to stimulate bone formation. LLLI stimulates proliferation of osteoblast precursor cells and cell differentiation and rhBMP-2 recruits osteoprogenitor cells to the bone healing area. This in vivo study evaluated the effects of LLLI and rhBMP-2 on the bone healing process in rats. Critical bone defects were created in the parietal bone in 42 animals, and the animals were divided into six treatment groups: (1) laser, (2) 7?μg of rhBMP-2, (3) laser and 7?μg of rhBMP-2, (4) 7?μg of rhBMP-2/monoolein gel, (5) laser and 7?μg rhBMP-2/monoolein gel, and (6) critical bone defect controls. A gallium-aluminum-arsenide diode laser was used (wavelength 780?nm, output power 60?mW, beam area 0.04?cm(2), irradiation time 80?s, energy density 120?J/cm(2), irradiance 1.5?W/cm(2)). After 15?days, the calvarial tissues were removed for histomorphometric analysis. Group 3 defects showed higher amounts of newly formed bone (37.89%) than the defects of all the other groups (P?
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