高氧诱导的视网膜新生血管模型鼠中血管生成素2及酪氨酸激酶受体表达的研究

目的研究高氧诱导的视网膜新生血管模型鼠中血管生成素2(Ang-2)及酪氨酸激酶(Tie-2)受体表达。方法高浓度氧诱导C57BL/6J小鼠建立视网膜缺血性病变动物模型。将30只小鼠分为2组:正常组;高氧组。视网膜切片HE染色,计数各组小鼠视网膜新生血管内皮细胞核数,并加以比较。于鼠龄17d取各组小鼠视网膜及前增生的血管膜,反转录聚合酶链反应(RT-PCR)方法半定量测定Ang-2及Tie-2受体mRNA的表达。结果吸入高氧鼠每只眼均可见突出内界膜的新生血管内皮细胞,发生率为100%。高氧组Ang-2及Tie-2受体mRNA表达分别为对照组的3.7倍(P<0.05)、4.1倍(P<0.05)。结论血管抑素能有效抑制高氧诱导的小鼠视网膜新生血管形成,但并不影响VEGF的合成和分泌,其抗血管新生作用可能为抑制VEGF功能发挥。

mRNA expression of angiopoietin-2 and tyrosinekinase receptor in experimental retinal neovascularization induced by oxygen

To test Expression of angiopoietin-2 (Ang-2) mRNA and tyrosinekinase receptor (Tie-2) mRNA in experimental retinal neovascularization induced by oxygen. Mouse models of hyperoxia-induced ischemic retinopathy were established. Mouse were divided into 2 groups: control and hyperoxic groups. The nuclei of new vessel buds extending from the retina into the vitreous in different groups were counted and compared under the light microscope. Fibrovascular tissues were obtained from 17 days-old mice in different groups. Reverse transcripition polymerase chain reaction (RT-PCR) analysis were performed to examine the expression of (Ang-2) mRNA and Tie-2 mRNA. There were plenty of new vessel buds in the eyes of all mice in hyperoxic condition. The mRNA expression of Ang-2 and Tie-2 were 3.7-fold increase and 4.1-fold relative to control cells respectively. [Conclusions] The upregulation of Ang-2 mRNA and Tie-2 mRNA plays a important role in retinal neovascularization.