| Nav1.1抗体对豚鼠心室肌细胞钠电流的影响 |
| |
| 引用本文： | 张涛,程龙献,雷鸣,史钰芳,刘坤,高翔,赵芳. Nav1.1抗体对豚鼠心室肌细胞钠电流的影响[J]. 中国心脏起搏与心电生理杂志, 2007, 21(3): 248-250 |
| |
| 作者姓名： | 张涛 程龙献 雷鸣 史钰芳 刘坤 高翔 赵芳 |
| |
| 作者单位： | 华中科技大学同济医学院附属协和医院心内科,湖北武汉,430022 |
| |
| 摘 要： | 目的探讨Nav1.1抗体对豚鼠心室肌细胞钠电流(INa)的影响。方法采用全细胞膜片钳技术,观察急性分离的豚鼠心室肌细胞在应用Nav1.1抗体后心肌INa的变化。结果在Nav1.1抗体作用下,钠离子通道电流密度呈浓度依赖性减小。用药前与三个浓度抗体组(1∶100,1∶50,1∶25)的电流密度分别为34.22±6.22,32.13±3.45,25.49±7.4,19.23±1.69pA/pF。其中1∶25Nav1.1抗体明显降低钠电流密度(P<0.05),并且,1∶25Nav1.1抗体使INa电流-电压曲线明显向正电位方向移动,但是其稳态失活曲线和恢复曲线没有显著性改变。结论Nav1.1可能对INa有影响。
|
| 关 键 词： | 电生理学 钠电流 心室肌细胞 抗体 |
| 文章编号： | 1007-2659（2007）03-0248-03 |
| 修稿时间： | 2006-10-19 |
The effect of antibody of Nav1.1 on sodium current in guinea pig ventricular cells |
| |
| ZHANG Tao,CHENG Long-xian,LEI Ming,SHI Yu-fang,LIU Kun,GAO Xiang,ZHAO Fang. The effect of antibody of Nav1.1 on sodium current in guinea pig ventricular cells[J]. Chinese Journal of Cardiac Pacing and Electrophysiology, 2007, 21(3): 248-250 |
| |
| Authors: | ZHANG Tao CHENG Long-xian LEI Ming SHI Yu-fang LIU Kun GAO Xiang ZHAO Fang |
| |
| Affiliation: | Department of Cardiology, The Affiliated Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430022, China |
| |
| Abstract: | Objective To study the effect of antibody of Nav1.1 on sodium current in guinea pig ventricular cells.Methods Detected the sodium current before and after applying antibody of Nav1.1(1∶100,1∶50,1∶25)to the isolated guinea pig ventricular cells with whole cell patch clamp recording.Results Antibody of Nav1.1 decreased the voltage activated Na+ currents in a concentration dependent manner.The density of Na+ current of control was 34.22 ± 6.22pA/pF,while those of test were 32.13 ± 3.45pA/pF,25.49 ± 7.4pA/pF,and 19.23 ± 1.69pA/pF respectively.Sodium current was inhibited by 1∶25 anti-Nav1.1 significantly.1∶25 anti-Nav1.1 shifted I-V relationship curve to more positive potentials.However,anti-Nav1.1 didn't change the steady-state recovery and inactivation of Na+ currents.Conclusions Sodium channel subtypes Nav1.1 contributes to the sodium current in ventricular cells. |
| |
| Keywords: | Electrophysiology Sodium current Ventricular cells, Antibody |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
