FHIT和p16基因甲基化与胃癌的关系研究

目的探讨FHIT和p16基因甲基化在胃癌发生发展中的作用。方法应用甲基化特异性PCR（MSP）,检测胃癌组织和正常胃黏膜组织中FHIT基因和p16基因的启动子CpG岛的甲基化状态。结果胃癌组织中FHIT和p16基因甲基化阳性率分别为51.4%和56.8%,两者的甲基化均与胃癌患者年龄、性别、Lauren分型、Borrmann分型、淋巴结转移以及TNM分期无关（P〉0.05）,且两者的甲基化不存在明显正相关关系（P〉0.05,γ=0.17）。结论 FHIT基因和p16基因的甲基化修饰在胃癌发生发展中均起重要作用,两者可能是胃癌发生的早期事件。

Study on The Methylation of FHIT and p16 Gene in Gastric Cancer Tissues

Objective To investigate the 5＇-CpG island aberrant methylation of fragile histidine triad（FHIT） and p16 gene promoter and to elucidate the role of gene methylation on tumorigenesis and progression of gastric carcinoma（GC）.MethodsMethylation of FHIT gene and p16 gene were detected with methylation specific PCR（MSP） in matched normal and cancer tissues from 74 patients with primary GC.Results Hypermethylation of FHIT gene and p16 gene were detectable in 51.4% and 56.8% in the GC tissues,respectively.Methylation expression of FHIT gene and p16 gene in GC was not correlated to age,gender,Lauren＇s classification,Borrmann＇s classification,lymphatic metastasis and TNM staging of tumor（P〉0.05）.There were no positive correlation between methylation of these two gene（P〉0.05,γ=0.17）.Conclusion Methylation of FHIT gene and p16 gene may play critical roles in the pathogenesis and progression of GC,and methylation of them may be anearly event in gastric carcinogenesis.