冠心病与NAD(P)H氧化酶p22phox基因C242T多态性

目的探讨烟酰胺腺嘌呤二核苷酸磷酸盐氧化酶NAD(P)H氧化酶]p22phox基因C242T多态性与冠心病的关系。方法采用病例对照研究方法,收集早发冠心病患者309例,迟发冠心病患者437例,对照470例,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)基因分型技术对p22phox基因C242T位点的多态性进行检测;采用单因素和多因素logistic回归计算p22phox基因C242T多态性与冠心病风险的OR值及95%CI。结果与对照组比较,早发冠心病组携带TC+TT基因型的个体比携带CC基因型更容易患冠心病,OR=1.58,95%CI=1.07～2.34;迟发冠心病组OR=1.48,95%CI=1.03～2.13;多因素调整后,与对照组比较,早发冠心病组OR=1.74,95%CI=1.16～2.62;2个病例组比较,携带TC+TT基因型与携带CC基因型的患病风险差异均无统计学意义。结论p22phox基因C242T多态性的T等位基因可能是冠心病的危险因素。

Relationship between coronary heart disease and NADH/NADPH oxidase p22phox gene C242T polymorphism

Objective To investigate the relationship between nicotinamide adenine dinucleotide hydrate/nicotinamide adenice dinucleotide phosphate hydrate(NADH/NADPH) oxidase p22phox gene C242T polymorphism and coronary heart disease(CHD).Methods A hospital-based case-control study was conducted among 309 premature CHD,437 late-onset CHD patients and 470 controls.Polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP) was used to detect the polymorphism.Univariate and multivariate logistic regression models were performed to estimate odds ratios(ORs) and 95% confidence interval(CI) of CHD.Results The odds ratios of CT+TT versus CC genotype of C242T polymorphisms for CHD between early-onset CHD group and controls were statistically significant in both univariate logistic model(OR=1.58,95%CI:1.07-2.34) and multivariate logistic model(OR=1.74,95%CI:1.16-2.62).For the comparison between late-onset CHD group and controls,the odds ratio was statistically significant(OR=1.48,95%CI:1.03-2.13) in univariate logistic model.However,when comparing early-onset CHD group with late-onset CHD group,the odds ratios in neither univariate analysis nor multivariate logistic regression were statistically significant.Conclusion T allele of p22phox gene C242T polymorphism may be a risk factor for CHD.