白藜芦醇对大鼠肾氧化损伤保护作用

目的 探讨白藜芦醇对高糖高脂饮食大鼠肾氧化损伤的保护作用。方法 大鼠分为正常对照组、高糖高脂组、白藜芦醇低、中、高剂量组,13周后处死大鼠,测定血糖血脂、肾组织氧化应激水平、抗氧化酶活性及肾脏过氧化物酶激活受体α(PPARα)、羟甲基戊二酸单酰辅酶A合成酶(HMGCS2)mRNA表达水平。结果 高糖高脂饮食13周后,高糖高脂组大鼠体重为(453.00±19.54)g,明显高于正常对照组(368.75±25.24)g和高剂量白藜芦醇组(400.40±30.39)g(t=4.545、2.991,P<0.05);高糖高脂组大鼠肾脏总抗氧化能力(T-AOC)、超氧化物歧化酶(T-SOD)分别为(1.22±0.34)、(2.69±0.13)U/(mg.Pro),明显低于正常对照组的(0.27±0.03)、(2.43±0.07)U/(mg.pro)(t=3.491,P=0.003;t=2.793,P=0.011)。结论 高糖高脂饮食大鼠肾内处于明显的氧化应激状态,白藜芦醇对高糖高脂饮食引起的氧化性肾损伤有保护作用。

Protective effects of resveratrol on renal oxidative injury in rats

Objective To examine the protective role of resveratrol on lipid oxidation which may cause kidney damage in rats with high glucose and high-fat diet.Methods The rats were divided into normal diet control group,high glucose and high-fat diet group,low,moderate and high dose resveratrol groups.All rats were killed after 13 weeks.Serum glucose,total cholesterol,and triglyceride levels were determined.Total antioxidant capacity(T-AOC),total superoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px) activity,and malondialdehyde(MDA) content were measured in renal tissue extracts and renal peroxisome proliferator-activated receptor alpha(PPARα),mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase(HMGCS2) mRNA expression were determined.Results Body weight(453.00±19.54 g),renal MDA(6.11±1.42 nmol/mg·Pro)content increased significantly in high glucose and high-fat diet group compared with normal diet control group,while decreased in resveratrol co-treated groups compared with high glucose and high-fat diet group.The renal T-AOC(0.27±0.03 U/mg·Por),T-SOD(2.43±0.07 U/mg·Pro)levels of high glucose and high-fat diet group were significantly lower than those of the normal diet control group.Compared with those of the high glucose and fat diet group,renal T-AOC,T-SOD,and GSH-Px levels decreased in resveratrol co-treated group.Compared with normal diet control group,lower renal PPARα and HMGCS2 mRNA expression levels were observed in high glucose and fat diet rats(P=0.012,0.045),while enhanced expression of renal PPARα and HMGCS2 mRNA were observed in the rats co-treated with resveratrol.Conclusion High glucose and high-fat diet can cause significant oxidative stress in rat kidney,which may be involved in the occurrence of renal damage.Resveratrol has protective effect on the high glucose and high-fat diet induced oxidative renal injury in rats.