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重组肿瘤坏死因子-α抗血清对大鼠实验性结肠炎的影响
引用本文:姜小丹,尹丙姣,石文芳,冯玮,徐勇,李卓娅. 重组肿瘤坏死因子-α抗血清对大鼠实验性结肠炎的影响[J]. 华中科技大学学报(医学版), 2005, 34(3): 281-283
作者姓名:姜小丹  尹丙姣  石文芳  冯玮  徐勇  李卓娅
作者单位:华中科技大学同济医学院基础医学院免疫学系,武汉,430030
基金项目:国家高技术研究发展计划(863计划)资助项目(No.2004AA215160)
摘    要:目的 探讨灌胃给予肿瘤坏死因子-α(TNF-α)多克隆抗体对溃疡性结肠炎的作用。方法 以50mg/kg三硝基苯磺酸(TNBS)经肛门注入大鼠肠腔,复制大鼠溃疡性结肠炎模型;用兔抗人TNF-α多克隆抗体血清10mg/kg给大鼠灌胃,观察一氧化氮(N0)含量、髓过氧化物酶(MPO)活性及TNF-α在大鼠溃疡性结肠炎组织中的表达。结果 抗TNF-α多克隆抗体治疗可明显减轻溃疡性结肠炎的病理改变,抑制局部结肠组织NO的产生、MPO活性以及TNF-α表达。结论 抗TNF-α血清通过中和TNF-α,可抑制溃疡性结肠炎结肠组织表达TNF-α及炎性介质的释放,从而减轻溃疡性结肠炎的病理损伤。

关 键 词:抗体,肿瘤坏死因子  溃疡性结肠炎  肿瘤坏死因子-α
修稿时间:2004-12-23

Application of Polyclonal Antibody Against Recombinant TNF-alpha on Experimental Rat Colonitis
Jiang Xiaodan,Yin Bingjiao,Shi Wenfang et al. Application of Polyclonal Antibody Against Recombinant TNF-alpha on Experimental Rat Colonitis[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2005, 34(3): 281-283
Authors:Jiang Xiaodan  Yin Bingjiao  Shi Wenfang et al
Affiliation:Jiang Xiaodan,Yin Bingjiao,Shi Wenfang et al Department of Immunology,School of Basic Medical Sciences,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030
Abstract:Objective To investigate the effect of polyclonal antiboidy against TNF on ulcer colonitis by stomach perfusion.Methods Experimental colonitis model was obtained by challenging the rat with 50 mg/kg TNBS with colon perfusion through anus. NO content, MPO activity and TNF-alpha expression in the ulcer colonitis tissue were determined.Results Polyclonal antibody against TNF-alpha reduced the pathological alternations of ulcer colonitis tissue, inhibited NO production, MPO activity and TNF-alpha expression as well.Conclusion Anti-TNF polyclonal antibody can inhibit the TNF-alpha expression and other inflammatory mediators by neutralizing TNF-alpha, which reduces pathological changes in local colonitis tissue and plays an important role in therapeutic effect.
Keywords:antibody  tunor necrosis factor-alpha  ulcer colonitis Crohn disease  tunor necrosis factor-alpha
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