| 重组肿瘤坏死因子-α抗血清对大鼠实验性结肠炎的影响 |
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| 引用本文： | 姜小丹,尹丙姣,石文芳,冯玮,徐勇,李卓娅.重组肿瘤坏死因子-α抗血清对大鼠实验性结肠炎的影响[J].华中科技大学学报(医学版),2005,34(3):281-283. |
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| 作者姓名： | 姜小丹 尹丙姣 石文芳 冯玮 徐勇 李卓娅 |
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| 作者单位： | 华中科技大学同济医学院基础医学院免疫学系,武汉,430030 |
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| 基金项目： | 国家高技术研究发展计划(863计划)资助项目(No.2004AA215160) |
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| 摘 要： | 目的 探讨灌胃给予肿瘤坏死因子-α(TNF-α)多克隆抗体对溃疡性结肠炎的作用。方法 以50mg／kg三硝基苯磺酸(TNBS)经肛门注入大鼠肠腔，复制大鼠溃疡性结肠炎模型；用兔抗人TNF-α多克隆抗体血清10mg／kg给大鼠灌胃，观察一氧化氮(N0)含量、髓过氧化物酶(MPO)活性及TNF-α在大鼠溃疡性结肠炎组织中的表达。结果 抗TNF-α多克隆抗体治疗可明显减轻溃疡性结肠炎的病理改变，抑制局部结肠组织NO的产生、MPO活性以及TNF-α表达。结论 抗TNF-α血清通过中和TNF-α，可抑制溃疡性结肠炎结肠组织表达TNF-α及炎性介质的释放，从而减轻溃疡性结肠炎的病理损伤。
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| 关 键 词： | 抗体，肿瘤坏死因子 溃疡性结肠炎 肿瘤坏死因子-α |
| 修稿时间： | 2004年12月23 |
Application of Polyclonal Antibody Against Recombinant TNF-alpha on Experimental Rat Colonitis |
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| Jiang Xiaodan,Yin Bingjiao,Shi Wenfang et al.Application of Polyclonal Antibody Against Recombinant TNF-alpha on Experimental Rat Colonitis[J].Journal of Huazhong University of Science and Technology(Health Sciences),2005,34(3):281-283. |
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| Authors: | Jiang Xiaodan Yin Bingjiao Shi Wenfang |
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| Institution: | Jiang Xiaodan,Yin Bingjiao,Shi Wenfang et al Department of Immunology,School of Basic Medical Sciences,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 |
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| Abstract: | Objective To investigate the effect of polyclonal antiboidy against TNF on ulcer colonitis by stomach perfusion.Methods Experimental colonitis model was obtained by challenging the rat with 50 mg/kg TNBS with colon perfusion through anus. NO content, MPO activity and TNF-alpha expression in the ulcer colonitis tissue were determined.Results Polyclonal antibody against TNF-alpha reduced the pathological alternations of ulcer colonitis tissue, inhibited NO production, MPO activity and TNF-alpha expression as well.Conclusion Anti-TNF polyclonal antibody can inhibit the TNF-alpha expression and other inflammatory mediators by neutralizing TNF-alpha, which reduces pathological changes in local colonitis tissue and plays an important role in therapeutic effect. |
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| Keywords: | antibody tunor necrosis factor-alpha ulcer colonitis Crohn disease tunor necrosis factor-alpha |
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