| C6／36细胞感染登革Ⅱ型病毒后基因表达的变化 |
| |
| 引用本文： | 杨发青,赵彤言,李德昌,董言德,陆宝麟. C6／36细胞感染登革Ⅱ型病毒后基因表达的变化[J]. 寄生虫与医学昆虫学报, 2001, 8(3): 155-158 |
| |
| 作者姓名： | 杨发青 赵彤言 李德昌 董言德 陆宝麟 |
| |
| 作者单位： | 1. 军事医学科学院微生物流行病研究所,
2. 国人民解放军军需大学, |
| |
| 基金项目： | 中国人民解放军总后勤部"九五”杰出中青年基金 |
| |
| 摘 要： | 本研究在C6/36细胞感染登革Ⅱ型病毒24h后,用DDRT-PCR对细胞基因表达的变化进行了研究,并对其中2条表达有变化的基因进行了序列分析,1条为感染后表达量增加的基因片段,1条为感染后表达量减少的基因片段,通过GenBank查询尚未发现同源序列.这一发现对于阐明登革病毒和其宿主细胞之间的关系,探讨病毒感染的细胞内机制有积极的意义.
|
| 关 键 词： | 登革病毒 C6/36细胞 DDRT-PCR |
| 修稿时间： | 2000-05-19 |
DIFFERENTIAL EXPRESSED GENES OF C6/36 CELLS INFECTED WITH DENGUE TYPE 2 VIRUSES |
| |
| Yang Faqing Zhao Tongyan Li Dechang Dong Yande Lu Baoling. DIFFERENTIAL EXPRESSED GENES OF C6/36 CELLS INFECTED WITH DENGUE TYPE 2 VIRUSES[J]. Acta Parasitologica et Medica Entomologica Sinica, 2001, 8(3): 155-158 |
| |
| Authors: | Yang Faqing Zhao Tongyan Li Dechang Dong Yande Lu Baoling |
| |
| Abstract: | Twenty four hours after C6/36 cells infected with Dengue type 2 viruses, Genes of C6/36 cells which alter their expression were examined by dfferential display PCR technique. Two gene fragments, one increasing its expression, the other decreasing its expression, were cloned and sequenced, respectively. No similar sequences were found in GenBank database. This finding would help for further understanding the interaction between Dengue virus and its host cell and for explaining the inner mechanism of dengue infection to its host cell. |
| |
| Keywords: | Dengue virus C6/36 cell DDRT PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
