| 苦参碱诱导K562细胞合成γ珠蛋白 |
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| 引用本文： | 张翠梅,封志纯.苦参碱诱导K562细胞合成γ珠蛋白[J].南方医科大学学报,2007,27(2):215-218. |
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| 作者姓名： | 张翠梅 封志纯 |
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| 作者单位： | 南方医科大学珠江医院儿科,广东,广州,510282;南方医科大学珠江医院儿科,广东,广州,510282 |
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| 基金项目： | 致谢：本实验在南方医科大学基础医学院细胞生物学教研室完成,陈平雁教授给予统计方面指导,谨致谢意! |
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| 摘 要： | 目的 体外研究苦参碱对K562细胞γ珠蛋白合成的影响.方法 以K562细胞为体外模型,采用联苯胺染色方法确定苦参碱诱导K562细胞血红蛋白表达增加的剂量效应和时间效应,进一步应用Western blotting技术检测血红蛋白F(αγ2)水平.结果 0.1mg/ml苦参碱作用于K562细胞,在96h联苯胺染色阳性细胞率达到15.67%;Western blotting检测到血红蛋白F表达增加.结论 苦参碱在体外可诱导K562细胞γ珠蛋白合成增加,从而使血红蛋白F增加.苦参碱具有与阳性对照丁酸钠相似的诱导γ珠蛋白合成增加的作用.
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| 关 键 词： | 苦参碱 γ珠蛋白 K562细胞 |
| 文章编号： | 1673-4254（2007）02-0215-04 |
| 收稿时间： | 2006-09-17 |
| 修稿时间： | 2006年9月17日 |
Matrine-induced gamma-globin synthesis in K562 cells |
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| ZHANG Cui-mei,FENG Zhi-chun.Matrine-induced gamma-globin synthesis in K562 cells[J].Journal of Southern Medical University,2007,27(2):215-218. |
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| Authors: | ZHANG Cui-mei FENG Zhi-chun |
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| Institution: | Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. zhangcuimei0527@163.com |
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| Abstract: | OBJECTIVE: To study the effects of matrine on gamma-globin synthesis in K562 cells in vitro. METHODS: Benzidine staining was used to study the dose- and time-dependent effects of matrine on hemoglobin synthesis in K562 cells, and Western blotting was performed to determine the level of hemoglobin F(alpha(2)gamma(2)). RESULTS: Benzidine staining showed that K562 cells treated with matrine of 0.1 mg/ml had a positivity rate for benzidine (BZ%) of 15.67% at 96 h after the treatment, and Western blotting indicated increased synthesis of hemoglobin F. CONCLUSION: Matrine can induce gamma-globin synthesis and increase hemoglobin F level in K562 cells, the effect of which resembles that of sodium butyrate. |
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| Keywords: | matrine gamma-globin K562 cells |
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