Fhit蛋白在人类胃组织中的表达意义

目的：探讨脆性组氨酸三联体（FHIT）基因产物Fhit蛋白在人类胃组织中的表达情况及其在胃癌发生发展过程中的可能意义。方法：采用免疫组化S-P法检测54例正常胃组织、31例癌旁正常组织、12例中重度不典型增生组织以及67例胃癌组织中Fhit蛋白的表达情况。结果：Fhit蛋白在胃癌组织中表达明显减少，与正常胃黏膜组织比较，差异十分显著（P〈0．01）：Fhit蛋白在胃癌不同组织学类型及Lauren分型中的表达差异十分显著（P〈0．01），而在不同TNM分期、淋巴结转移级别等分组指标中的表达差异无统计学意义（P〉0．05）；Fhit蛋白在正常胃黏膜组织、良性胃病组织、中重度不典型增生及胃癌4组中表达下降或缺失率逐渐升高，差异十分显著（P〈0．01）。结论：Fhit蛋白在弥漫型、分化不良型胃癌中丢失显著，提示可能预后不佳：Fhit蛋白表达下降或缺失可能与胃癌发生过程相关。

Significance of Fhit protein expression in human gastric tissue

Objective: To investigate the expression of the Fhit protein,the fragile histidine triad(FHIT) gene product,in human gastric tissues and its possible significance in the tumorigenesis and development of gastric cancer(GC).Methods: Fhit protein expression was detected by immnohistochemical staining in 54 normal gastric tissues,31 cases of premalignant disease,12 cases of moderate or severe dysplasia,and 67 cases of GC.Results: The positive expression rate of Fhit protein in patients with GC was significantly lower than that in normal gastric tissues(P < 0.01).There was significant difference in the positive expression rate of Fhit protein among patients with different histological or Lauren types of GC(P < 0.01),but no difference was found among patients with different TNM stages of GC(P > 0.05).The positive expression rate of Fhit protein was reduced in turn from normal gastric tissues to premalignant diseases to moderate or severe dysplasia to GC,and the differences in the positive expression rate of Fhit protein among these 4 groups were significant(P < 0.01).Conclusion: The loss of Fhit protein expression was notable in diffuse type and poorly differentiated type of GC,which may suggest the worse prognosis,and the decreased Fhit protein expression and loss of Fhit protein expression may play an important role in the tumorigenesis and development of GC.