高同型半胱氨酸血症对平滑肌细胞的氧化损伤

目的观察同型半胱氨酸血症对兔颈动脉平滑肌细胞氧化损伤,进而从细胞及分子水平探讨同型半胱氨酸血症对活体动物平滑肌细胞的氧化损伤的发病机制。方法以高蛋氨酸饮食饲喂新西兰大白兔,3个月后分离颈动脉,分离得到的兔颈动脉平滑肌用胰蛋白酶消化处理,取消化后的细胞进行培养。采用黄嘌呤氧化比色法测定细胞内超氧化物歧化酶(superoxidedismutase,SOD)的活力。用可见光比色法测定细胞内过氧化氢(catalase,CAT)的活力。结果在高蛋氨酸饮食作用下,兔颈动脉平滑肌细胞内CAT、SOD的含量明显增高,与2%的蛋氨酸低饲喂浓度及对照组比较,差异有统计学意义(P<0.05)。结论同型半胱氨酸血症可加速动脉血管壁平滑肌细胞的增生,并可能在一定剂量范围内造成动脉血管壁平滑肌细胞的氧化损伤加重,使机体动脉粥样硬化的进程加速。

Damage of hyperhomocysteinemia on generation oxidation in smooth muscle cells

Objective Many recent studies have indicated that homocysteine(Hcy) is an important and independent risk factor for atherosclerosis(AS). This study aimed to test the effects of Hcy on the vascular smooth muscle cells in rabbits' carotid artery by feeding New Zealand white rabbits with high concentration of Hcy. Methods The carotid artery was separated after feeding with high methionine foods for three months. The vascular smooth muscle cells(VSMCs) in rabbits' carotid artery were separated, cultured and passaged. The intracellular superoxide dismutase(SOD) activity was tested using the xanthine oxidation.The intracellular activity of catalase(CAT) was measured by visible colorimetric method. Results The pathomorphological change of the separated rabbit carotid artery with methionine diet group was compared with control group under optical microscope. I: Intima; M: Medial. The CAT and SOD activity of rabbit carotid artery smooth muscle cells increase under Hcy diet group. Conclusion High Hcy accelerated VMSC proliferation, and may increase peroxidizine damages of VMSCs within the scope of certain dose, eventually accelerate the process of AS.