超高效液相色谱法同时测定知母中芒果苷等6种成分的含量

 目的 建立同时测定知母中新芒果苷,芒果苷,知母皂苷BⅡ,2,6,4′-三羟基-4-甲氧基苯甲酮,构树宁B,顺-扁柏树脂酚的UPLC方法。方法 采用ACQUITY UPLC, HSS T3色谱柱(2.1 mm×100 mm, 1.8 μm),流动相为乙腈-0.03%的磷酸水溶液梯度洗脱,流速为0.5 mL·min^-1,检测波长采用210和192 nm。结果 新芒果苷,芒果苷,知母皂苷BⅡ,2,6,4′-三羟基-4-甲氧基苯甲酮,构树宁B,顺-扁柏树脂酚的质量浓度与峰面积分别在15.00～299.6 μg·mL^-1, 8.80～175.6 μg·mL^-1, 40.00～800.0 μg·mL^-1,2.300～45.60 μg·mL^-1, 0.420～8.50 μg·mL^-1, 0.400～9.00 μg·mL^-1内呈良好的线性关系。平均加样回收率（n=9）均在97.7%~100.6%之间,RSD均小于1.2%。结论 该方法快速、准确、简便,可作为同时测定知母中新芒果苷,芒果苷,知母皂苷BⅡ,2,6,4′-三羟基-4-甲氧基苯甲酮,构树宁B,顺-扁柏树脂酚的方法。

UPLC Determination of Six Active Components Including Mangiferin in Anemarrhenae Rhizoma

OBJECTIVE To establish an analytical method to determine 7-O-glucopyranosylmangiferin, mangiferin, timosaponinBⅡ, 2,6,4′-trihydroxy-4-methoxybenzophenone, broussonin B and cis-hinokiresinol in Anemarrhenae Rhizoma quickly and accurately. METHODS A UPLC method was established on an HSS T3 column (2.1 mm×100 mm, 1.8 μm). The mobile phase consisted of 0.03% phosphoric acid solution and acetonitrile eluted in gradient mode, and the detection wavelength was set at 192 and 210 nm. RESULTS The standard curves of 7-O-glucopyranosylmangiferin, mangiferin, timosaponin BⅡ, 2, 6, 4′-trihydroxy-4-methoxybenzophenone, broussonin B and cis-hinokiresinol showed good linearity in 15.00-299.6 μg·mL^-1, 8.80-175.6 μg·mL^-1, 40.00-800.0 μg·mL^-1, 2.300-45.60 μg·mL^-1, 4.200-8.50 μg·mL^-1 and 0.400-9.00 μg·mL^-1, respectively. The mean recoveries (n=9)of the 6 components were 97.7%-100.6% with RSD of 1.2%. CONCLUSION The method is quick and accurate and can be used to determine 7-O-glucopyranosylmangiferin, mangiferin, timosaponin BⅡ, 2,6,4′-trihydroxy-4-methoxybenzophenone, broussonin B and cis-hinokiresinol in Anemarrhenae Rhizoma.