基于中红外光谱技术快速测定玄参生品及炮制品中哈巴俄苷含量的研究

 目的 采用中红外光谱衰减全反射光谱技术(attenuated total reflectance，ATR) 对玄参生品与炮制品中哈巴俄苷的含量进行快速无损的测定。方法 以高效液相色谱为对照分析方法，测定玄参炮制前后哈巴俄苷的含量，运用偏最小二乘法（partial least squares，PLS）建立ATR光谱与哈巴俄苷的HPLC分析值之间多元校正模型，并对炮制前后的未知样本进行预测。结果 校正模型决定系数生品为r²=83.81，SEE和SEP分别为0.021 55和0.051 03；制品校正模型决定系数为r²=95.56，SEE和SEP分别为0.007 30和0.034 78。 结论 该法可以作为一种准确、快速、高效、无损的检测方法用于检测中药炮制前后有效成分的变化规律。

Rapid Determination of Harpagoside Content in Crude and Processed Radix Scrophulariae by Mid-Infrared Spectroscopy

OBJECTIVE To investigate the feasibility of using mid-infrared spectroscopy (MIRS) technology for rapid quantification of harpagoside contents in crude and processed Radix Scrophulariae by means of attenuated total reflectance. METHODS HPLC was used as the reference method to determine the content of harpagoside，and multivariate calibration models based on PLS algorithm were developed to correlate the spectra and the corresponding values determined by the reference method. RESULTS The determination coefficient of the calibration model for crude Radix Scrophulariae was r²=83.81，and the standard error of estimate (SEE) and the standard error of prediction (SEP) were 0.021 55 and 0.051 03，respectively. The determination coefficient of the calibration model for processed Radix Scrophulariae was r²=95.56，and the standard error of estimate (SEE) and the standard error of prediction (SEP) were 0.007 30 and 0.034 78， respectively. CONCLUSION The proposed method is fast and nondestructive，and it provides a novel efficient and environmental friendly approach for the fast determination of the active components in crude and processed traditional Chinese medicine (TCM).