c-Met在食管鳞状细胞癌组织中的表达及其与临床病理的相关性

目的检测食管鳞状细胞癌组织及配对癌旁组织中c-Met蛋白的表达情况,探讨c-Met的表达与食管鳞状细胞癌患者临床病理特点的相关性。方法收集87对甲醛固定,石蜡包埋的食管鳞状细胞癌及配对的癌旁组织标本,所有病例均经病理证实,同时收集患者的临床资料。采用免疫组化的方法检测组织中c-Met的表达情况。结果 c-Met主要表达于细胞膜,表现为胞膜呈棕黄色染色。87例食管鳞癌组织中有29例(33.3%)呈阳性表达。配对癌旁组织中未见c-Met表达。c-Met的表达与食管鳞状细胞癌患者的肿瘤浸润深度、淋巴结转移及临床病理分期(tumor-node-metastasis,TNM)存在显著相关性(分别为P=0.017,P=0.000,P=0.000),而与患者的性别、年龄、肿瘤细胞分化程度、肿瘤大小等无明显相关性(P>0.05)。结论 c-Met在部分食管鳞癌组织中呈高表达,且与患者的肿瘤浸润深度、淋巴结转移及TNM分期存在显著相关性,c-Met的高表达可以作为高侵袭性食管鳞癌的分子标志物。

c-Met expression in esophageal squamous cell carcinoma tissues and its correlation with clinicopathological characteristics

Objective To detect the expression of c-Met in esophageal squamous cell carcinoma tissues(ESCC) and paired non-cancerous tissues, and to investigate the association between c-Met and clinicopathological parameters of ESCC patients. Methods Eighty seven pairs of paraffin-embedded ESCC tissues and paired non-cancerous esophageal tissues were collected from the Department of Pathology, Beijing Friendship Hospital Affiliated To Capital Medical University. Sections from each specimen were examined by a pathologist and graded histologically. Patients' clinical parameters were collected meanwhile. Immunohistochemistry(IHC) was applied to detected the the expression of c-Met in the tissues. Results c-Met positive signals showed brown-yellow granules in the membrane. The expression of c-Met in non-cancerous esophageal tissues was always negative, whereas strong positive staining of c-Met was observed in 29/87(33.3%) of ESCCs. The expression of c-Met was correlated with tumor invasion depth, lymph node metastasis, and TNM(tumor-node-metastasis) stage(P=0.017, P=0.000, P=0.000, respectively). No significant association between c-Met expression and other clinicopathological parameters(patients' gender, age, cell grading, and tumor size) was found(P>0.05). Conclusion The overexpression of c-Met was correlated with tumor invasion depth, lymph node metastasis, and TNM stage of ESCC patients. The overexpression of c-Met may be a marker for highly invasive ESCC.