子宫腺肌病PGP9.5、NGF的表达及其与痛经的关系

目的研究蛋白基因产物9.5(protein gene product 9.5,PGP 9.5)、神经生长因子(nerve growth factor,NGF)在子宫腺肌病(adenomyosis,ADS)在位内膜功能层和腺肌病灶中的表达及其与痛经的关系,探讨神经纤维及其生长因子在子宫腺肌病痛经中的作用。方法采用免疫组化SP法,检测ADS在位内膜功能层和病灶组织,对照组在位内膜功能层、肌层组织中PGP 9.5、NGF的表达;通过视觉模拟评分法(visual analogue scale/score,VAS)对ADS痛经程度评分,并分析其与上述因子的相关性。结果 PGP 9.5在ADS在位子宫内膜功能层中呈特异性表达;与对照组相比,PGP 9.5、NGF的表达在ADS在位内膜功能层和病灶中明显增高(P<0.05);ADS在位内膜功能层和ADS腺肌病灶中PGP 9.5、NGF的表达与痛经程度有显著相关性,差异有统计学意义(P<0.05),r分别为0.520和0.688,0.543和0.503。结论 ADS在位内膜功能层中PGP 9.5的特异性表达,可能为ADS诊断提供新的途径;ADS中PGP 9.5、NGF的高表达与其痛经程度呈正相关。

Expression of PGP 9.5,NGF and their relationship with dysmenorrhea in patients with adenomyosis

Objective To study the expression of nerve fibers marked by protein gene product 9.5 (PGP 9.5) and nerve growth factor (NGF) in situ function layer and ectopic endometrium of adenomyosis (ADS), the relation between the expression of PGP 9.5, NGF and NOGO-B and dysmenorrhea, and the role of nerve fibers in adenomyosis and dysmenorrhea.Methods Thirty ADS patients were enrolled and the expression of PGP 9.5, NGF and in in situ function layer and ectopic endometrium were determined by immunohistochemical method. Normal endometrial tissue from 30 individuals and myometrium from 27 individuals was included as the control group. The relationship of dysmenorrhea score of ADS patients by visual analogue scales (visual analogue scale/score, VAS) and the above-mentioned factors were analyzed.Results In in situ endometrial function layer, PGP 9.5 was specifically expressed in ADS patients. The expressions of PGP 9.5, NGF in ADS in situ function layer and ectopic endometrial tissue were stronger.than those of control group(P<0.05). There was significant correlation between the expression of PGP 9.5, NGF and the degree of dysmenorrhea of ADS-E group (P<0.05), r=0.520, 0.688. The same with the expression of PGP 9.5 and NGF and the degree of dysmenorrhea of ADS-M group (P<0.01), r=0.543 and 0.503. Conclusion The special expression of PGP 9.5 in endometrial function layer of adenomyosis suggests that PGP 9.5 may be a new factor to diagnose adenomyosis. The high expression of PGP 9.5, NGF in adenomyosis suggested that the factors mentioned are positively correlated with dysmenorrhea.