葫芦素B固体脂质纳米粒对人神经母细胞瘤SK-N-SH细胞的体外细胞毒作用评价

目的研究葫芦素B固体脂质纳米粒对人神经母细胞瘤SK-N-SH细胞的体外细胞毒性。方法采用四甲基偶氮唑蓝(MTT)法检测葫芦素B固体脂质纳米粒对人神经母细胞瘤SK-N-SH的细胞毒性;倒置显微镜观察细胞形态;流式细胞仪定量分析细胞周期。结果作用48 h后,葫芦素B以剂量依赖方式抑制SK-N-SH细胞的生长,0.143和0.716μmol.L-1葫芦素B固体脂质纳米粒对SK-N-SH细胞的抑制率分别为(34.9±4.6)%和(53.6±6.3)%,与对应浓度的葫芦B原料组的(13.2±2.3)%和(43.4±5.5)%相比较有显著性差异(P<0.05)。葫芦素B固体脂质纳米粒对人神经母细胞瘤SK-N-SH细胞的IC50为0.508μmol.L-1,而葫芦素B原料为12.6μmol.L-1。细胞形态观察结果显示,与二甲基亚砜(DMSO)组相比较,葫芦素B原料及葫芦素B固体脂质纳米粒组的细胞密度下降,细胞体积缩小,大多数细胞形状发生改变。流式细胞仪检测结果显示,葫芦素B原料可将SK-N-SH细胞抑制在S期和G2/M期,而葫芦素B固体脂质纳米粒可把SK-N-SH抑制在G2/M期。结论葫芦素B固体脂质纳米粒与葫芦素B原料药既能抑制人神经母细胞瘤SK-N-SH细胞增殖,又能诱导其凋亡,显示出较强的细胞毒性,且前者毒性更强。

Cytotoxicity Evaluation of Cucurbitacin B Solid Lipid Nanoparticles (SLN) on Human Neuroblastoma SK-N-SH Cell Line in Vitro

OBJECTIVE To evaluate the cytotoxicity of cucurbitacin B solid lipid nanoparticles(SLN) on human neuroblastoma SK-N-SH cell line in vitro. METHODS The SK-N-SH cell line was treated with cucurbitacin B SLN at various concentrations. Growth suppression was evaluated by MTT method； apoptosis related alterations in morphology were ascertained under light microscopy. Flow cytometry(FCM) was used to investigate the distribution of cell life. RESULTS Free cucurbitacin B and cucurbitacin B SLN inhibited the growth of SK-N-SH cells after 48 h treatment in a dose dependent manner， with IC₅₀ values of 0.508 and 12.6 μmol·L^-1 ， respectively. The apoptotic rates at concentrations of 0.143 and 0.716 μmol·L^-1 were （34.9±4.6）% and（53.6±6.3）%， respectively，all of which were significantly higher than that of free cucurbitacin B （13.2±2.3）% and（43.4±5.5）% (P