应用亚硫酸钠快速制备低氧性肺血管收缩离体模型所需低氧性Krebs-Henseleit液的方法及评价

 目的：探讨应用亚硫酸钠（sodium sulfite, Na₂SO₃）快速去氧制备低氧性肺血管收缩（hypoxia pulmonary vasoconstriction, HPV）离体模型所需低氧性Krebs-Henseleit（KH）溶液的可行性。方法：在暴露于空气的开放环境中将不同剂量Na₂SO₃加至0.5 L 温度为37 °C的KH溶液中，用i-STAT便携式临床分析仪检测加样1 min后溶液pH值、氧分压（PO₂）、二氧化碳分压（PCO₂）和钠离子（Na⁺）浓度的变化；选择适合HPV模型条件的Na₂SO₃剂量溶入0.5 L KH液后，检测此低氧溶液在1、10、20、30、60和90 min时上述指标的变化。结果：大于0.2 g（包括0.2 g）Na₂SO₃加至0.5 L KH液中可降低KH液的PO₂（P<0.01），且随着剂量的增加，PO₂降低。其中，1.5 g Na₂SO₃剂量可使0.5 L KH液达到HPV模型所需的低氧状态PO₂（20~40 mmHg）并维持此低氧状态至少90 min，溶液pH值、PCO₂和Na⁺浓度均处于正常范围。结论：应用Na₂SO₃可制备HPV离体模型所需的低氧溶液，方法简单，快速可行，状态稳定，无需密闭。

Rapid preparation of hypoxic Krebs-Henseleit solution with sodium sulfite for in vitro model of hypoxic pulmonary vasoconstriction

AIM: To investigate the feasibility of using sodium sulfite (Na₂SO₃) to prepare hypoxic Krebs-Henseleit (KH) solution for hypoxic pulmonary vasoconstriction (HPV) model in vitro. METHODS: Different doses of Na₂SO₃ were added into 0.5 L KH solution at 37 °C. An i-STAT portable clinical analyzer was used to measure the oxygen partial pressure (PO₂), carbon dioxide partial pressure (PCO₂), pH value and the concentration of sodium (Na⁺) in these KH-Na₂SO₃ solutions 1 min after administration. Then the dose of Na₂SO₃ suitable for HPV model was dissolved in 0.5 L KH solution and the above indexes in the solution were monitored at various time points at 37 °C under atmospheric pressure. RESULTS: More than 0.2 g (including 0.2 g) Na₂SO₃ reduced the PO₂ of 0.5 L KH solution in a dose-dependent manner (P<0.01). In addition, 1.5 g Na₂SO₃ reduced the PO₂ of 0.5 L KH solution to 20~40 mmHg and maintained the hypoxic state for at least 90 min (suitable for HPV model in vitro), but had nearly no effect on the PCO₂, pH and Na⁺ levels. CONCLUSION: The hypoxia solution for HPV model could be reached by Na₂SO₃ in open air and the method is simple, easily feasible and stable.