| 整合素αvβ6对结肠癌细胞中整合素αvβ5内吞胞吐循环的影响 |
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| 引用本文： | 张琦,王健,陈融,彭程,牛卫博,牛军. 整合素αvβ6对结肠癌细胞中整合素αvβ5内吞胞吐循环的影响[J]. 中国病理生理杂志, 2012, 28(12): 2154-2159. DOI: 10.3969/j.issn.1000-4718.2012.12.008 |
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| 作者姓名： | 张琦 王健 陈融 彭程 牛卫博 牛军 |
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| 作者单位： | 山东大学第二医院血液肿瘤生物治疗研究所,山东 济南 250033;浙江大学医学院附属第二医院肿瘤外科,浙江 杭州 310009;山东大学 医学院机能实验室,齐鲁医院肝胆外科,山东 济南 250012 |
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| 基金项目： | 国家自然科学基金资助项目（No. 30570833; No. 30872460） |
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| 摘 要： | 目的：探讨整合素αvβ6对结肠癌细胞中整合素αvβ5内吞胞吐循环及细胞黏附、迁移能力的影响。方法：采用Western blotting检测不同细胞中整合素αvβ6和αvβ5的表达情况,通过整合素内吞实验、胞吐实验和capture-ELISA实验检测不同细胞中整合素αvβ6和αvβ5的内吞胞吐循环时相,利用细胞黏附实验和细胞迁移实验检测各种细胞在不同基质表面黏附和迁移能力的差异。结果：SW480、SW480 wild-type β6和SW480 mock细胞中整合素αv亚基和β5亚基的表达无显著差异（P>0.05）,SW480 wild-type β6细胞中整合素β6亚基的表达显著高于另外2种细胞（P<0.05）;SW480细胞中整合素αvβ5存在内吞胞吐循环,但当向SW480细胞中转染β6亚基后,整合素αvβ6进行内吞胞吐循环的同时,会对整合素αvβ5的内吞和胞吐过程产生抑制作用,差异有统计学意义（P<0.05）;3种细胞中整合素αvβ6和αvβ5的内吞胞吐循环的差异,会影响细胞在纤连蛋白（整合素αvβ6配体）和玻连蛋白（整合素αvβ5配体）表面的黏附和迁移能力。结论：整合素αvβ6和αvβ5拥有共同的α亚基,它们在细胞内的内吞胞吐循环存在某种竞争性关系,当二者同时存在时,整合素αvβ6会抑制αvβ5的内吞胞吐循环过程,并由此对细胞在相应基质表面黏附和迁移能力产生影响。
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| 关 键 词： | 整合素类 结肠肿瘤 胞吞作用 胞吐作用 细胞运动 |
| 收稿时间： | 2012-07-19 |
Effects of integrin αvβ6 on endo-exocytotic cycle of integrin αvβ5 in colon cancer cells |
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| ZHANG Qi,WANG Jian,CHEN Rong,PENG Cheng,NIU Wei-bo,NIU Jun. Effects of integrin αvβ6 on endo-exocytotic cycle of integrin αvβ5 in colon cancer cells[J]. Chinese Journal of Pathophysiology, 2012, 28(12): 2154-2159. DOI: 10.3969/j.issn.1000-4718.2012.12.008 |
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| Authors: | ZHANG Qi WANG Jian CHEN Rong PENG Cheng NIU Wei-bo NIU Jun |
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| Affiliation: | Institute of Biotherapy for Hematological Malignancies, the Second Hospital of Shandong University, Jinan 250033, China; Department of Surgical Oncology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China; Teaching Laboratory of Functional Science, School of Medicine, Department of Hepatobiliary, Qilu Hospital, Shandong University, Jinan 250012, China. |
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| Abstract: | AIM: To investigate the effects of integrin αvβ6 on the endo-exocytotic cycle of integrin αvβ5 in colon cancer cells. METHODS: The expression of integrin αvβ6 and αvβ5 in SW480 cells, SW480 wild-type β6 cells and SW480 mock cells was detected by Western blotting. The trafficking of integrin αvβ6 and αvβ5 was examined by endocytosis assay, exocytosis assay and capture-ELISA. The adhesive and migration abilities of these 3 cell lines towards fibronectin or vitronectin were measured by cell adhesion assay and cell migration assay. RESULTS: The expression of integrin αv and β5 subunits was similar in these 3 cell lines (P>0.05), while the expression of integrin β6 subunit in SW480 wild-type β6 cells was much higher than that in the other 2 cell lines (P<0.05). The transfection of integrin β6 subunit into SW480 cells was able to slow down the endocytosis and exocytosis of integrin αvβ5, and subsequently inhibited the cellular adhesion and migration abilities towards vitronectin. CONCLUSION: Integrin αvβ6 and αvβ5 share the same αv subunit. In the process of endocytosis and exocytosis, there might be some competitive relationship between these 2 integrin isoforms. Compared with integrin αvβ5, integrin αvβ6 has the priority to get into the trafficking process, and it has subsequent effects on the adhesion and migration of colon cancer cells. |
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| Keywords: | Integrins Colonic neoplasms Endocytosis Exocytosis Cell movement |
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