三氧化二砷联合顺铂对人类非小细胞肺癌细胞生长及凋亡抑制基因XIAP表达的影响

背景与目的 在中国肺癌居各种肿瘤之首位,寻求肺癌有效的治疗方法已成为人们关注的热点.本研究探讨三氧化二砷(As2O3)注射液与顺铂(DDP)联合应用对人类非小细胞肺癌细胞的生长、细胞凋亡及其X染色体连锁的凋亡抑制蛋白(XIAP)的影响.方法 采用四甲基偶氮唑蓝(MTT)比色法观察As2O3注射液和/或DDP对人类非小细胞肺癌细胞H460生长的影响;应用流式细胞术观察As2O3注射液和/或DDP处理前后细胞凋亡率;应用半定量RT-PCR检测As2O3注射液和/或DDP处理前后H460细胞中XIAP mRNA表达变化.结果 与单药作用相比,As2O3与DDP联合应用可显著抑制H460的增殖,增加细胞的凋亡率,并对细胞XIAP mRNA表达有增强抑制的作用.结论 与单药相比较,As2O3注射液联合DDP能够明显提高非小细胞肺癌对化疗的敏感性,其机制可能与抑制非小细胞肺癌的凋亡抑制蛋白XIAP的表达有关.

The influence of arsenic trioxide combined with cisplatin on the growth and expression of X-linked inhibitor of apoptosis protein, XIAP of human non-small cell lung cancer cells

Background and objective Lung cancer is the leading cancer of malignant tumors in China. It is the direction that people make effect to seek effective therapy of lung cancer. The aim of this study is to investigate the influence of arsenic trioxide (As2O3) injection combined with cisplatin (DDP) on the growth, apoptosis and XIAP mRNA of human non-small cell lung cancer cell lines. Methods The influence of growth of human non-small cell lung cancer cell lines H460 induced by As2O3 combined with DDP or without DDP was analysed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The apoptosis rate of H460 was measured by flow cytometry. XIAP mRNA level of the cells was detected by RT-PCR before and after treatment of As2O3 combined with DDP or without DDP. Results Compared to individual drug, As2O3 injection combined with DDP may obviously inhibit the proliferation of H460 cells, increase the apotosis rate of cells and strengthen inhibition of XIAP mRNA expression in cells. Conclusion As2O3 injection combined with DDP can significantly increase the chemosensitivity on human non-small cell lung cancer, and the mechanism may be related to its inhibition on the expression of XIAP.