| 细胞色素P450基因对高脂饮食诱导不同周龄小鼠动脉粥样硬化的保护作用 |
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| 引用本文： | 吴俊,陈琛,曾和松,汪道文.细胞色素P450基因对高脂饮食诱导不同周龄小鼠动脉粥样硬化的保护作用[J].中国分子心脏病学杂志,2011,11(1):37-41. |
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| 作者姓名： | 吴俊 陈琛 曾和松 汪道文 |
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| 作者单位： | 1. 长江航运总医院检验科
2. 华中科技大学同济医学院附属同济医院心血管内科,武汉市解放大道1095# |
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| 摘 要： | 目的研究血管内皮高表达CYP2C8基因能否改善小鼠动脉粥样硬化。方法以20和40周龄APOEKO+/-CYP2C8Tg+/-和CYP2C8Tg+/-基因(血管内皮特异性CYP2C8+/-转基因)的小鼠(n=10/组)为研究对象,相同基因背景和周龄的同窝APOEKO+/-和C57BL/6小鼠设为对照。采用PCR技术鉴定小鼠CYP2C8+/-基因;油红O染色检测APOEKO+/-CYP2C8Tg+/-、CYP2C8Tg+/-、APOEKO+/-和C57BL/6小鼠主动脉斑块形成面积;酶比色检测APOEKO+/-CYP2C8Tg+/-、CYP2C8Tg+/-、APOEKO+/-和C57BL/6小鼠血清TG、TCH、HDL。结果 20和40周龄APOEKO+/-CYP2C8Tg+/-和CYP2C8Tg+/-小鼠主动脉斑块形成面积与同周龄野生型小鼠相比明显减少,并且明显改善了血脂代谢状态。结论高表达CYP2C8基因能够改善西方饮食诱导的不同周龄小鼠的动脉粥样硬化。
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| 关 键 词： | 细胞色素P450 环氧化二十碳三烯酸 动脉粥样硬化 |
Effects of Arachidonic Acid Cytochrome P450 Epoxygenase on Atherosclerosis Induced by Western Diet in Different Week-old Mice Model |
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| WU Jun,CHEN Chen,ZENG He-Song,WANG Dao-Wen.Effects of Arachidonic Acid Cytochrome P450 Epoxygenase on Atherosclerosis Induced by Western Diet in Different Week-old Mice Model[J].Molecular Cardiology of China,2011,11(1):37-41. |
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| Authors: | WU Jun CHEN Chen ZENG He-Song WANG Dao-Wen |
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| Institution: | .Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030 ,China,* General Hospital of the Yangtze River Shipping,Wuhan,430010,China |
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| Abstract: | Objective To study whether overexpression of arachidonic acid cytochrome P450 (CYP) 2C8 epoxygenase genes improve Atherosclerosis in mice.Methods Overexpression CYP2C8 genes in endothelium of 20-week-old and 40-week-old of APOEKO+/- CYP2C8Tg+/-and CYP2C8Tg+/- mice were identified using PCR. The same aged APOEKO+/- and C57BL/6 mice were served as control (n=10). When the mice were sacrificed at 20 and 40 week of age, the index of atherosclerotic formation were measured by oil red O. Samples were used to meas... |
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| Keywords: | Arachidonic Acid Cytochrome P450 Epoxygenase Epoxyeicosatrienoic Acids Atherosclerosis |
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