| 芍药苷对心脏钾通道电生理功能的影响 |
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| 引用本文： | 王蓉蓉,王可,李宁,冉玉琴,浦介麟.芍药苷对心脏钾通道电生理功能的影响[J].中国分子心脏病学杂志,2011,11(1). |
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| 作者姓名： | 王蓉蓉 王可 李宁 冉玉琴 浦介麟 |
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| 作者单位： | 北京协和医学院中国医学科学院阜外心血管病医院病理与生理中心,北京,100037 |
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| 摘 要： | 目的研究芍药苷对内向整流钾电流(IK1)、瞬时外向钾电流(Ito)以及延迟整流钾电流(IKs和IKr)的作用。方法用全细胞膜片钳技术记录大鼠心室肌细胞的Ito和IK1电流。而IKs和IKr电流在转染相应质粒的HEK293细胞上记录。对比芍药苷使用前后的电流图,观察芍药苷对各种离子通道电流的影响。结果在-100mV测试电压下,100μmol/L的芍药苷能使IK1峰值密度从(-25.26±8.21)pA/pF降至(-17.65±6.52)pA/pF,平均抑制率为30.13%(n=6,P<0.05),但对其反转电位以及内向整流特性无影响。此外,100μmol/L芍药苷对Ito、IKs和IKr电流无明显作用。结论芍药苷对IK1电流具有明显的抑制作用,而对Ito、IKs及IKr无明显作用。
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| 关 键 词： | 心脏钾通道 中药 抗心律失常药 芍药苷 |
The effects of paeoniflorin monomer on cardiac potassium currents. |
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| WANG Rong-rong,WANG Ke,LI Ning,RAN Yu-qin,PU Jie-Iin.The effects of paeoniflorin monomer on cardiac potassium currents.[J].Molecular Cardiology of China,2011,11(1). |
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| Authors: | WANG Rong-rong WANG Ke LI Ning RAN Yu-qin PU Jie-Iin |
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| Institution: | WANG Rong-rong; WANG Ke; LI Ning; RAN Yu-qin; PU Jie-lin; Pathology and Physiology Research Center; Cardiovascular Institute and Fu Wai Hospital; Peking Union Medical College and Chinese Academy of Medical Sciences; Beijing 100037; China; |
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| Abstract: | Objective The present study was to determine the effects of paeoniflorin on inward rectifier potassium current (IK1), transient outward potassium current (Ito), and delayed rectifier current (IKs and IKr). Methods Whole-cell patch-clamp technique was used to record ion channel currents. IK1 and Ito were studied in rat ventricular myocytes; and IKs and IKr were investigated in transfected HEK293 cells. Results 100μmol/L paeoniflorin inhibited the IK1 by 30.13% at the test potential of -100mV (from (-25.26±8.... |
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| Keywords: | Potassium currents Chinese herbs Antiarrhythmic drugs Paeoniflorin |
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