葡萄籽原花青素对2型糖尿病大鼠血清中SOD GSH MDA及NO表达的影响

目的观察葡萄籽原花青素(GSPE)对2型糖尿病大鼠血清中超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)、丙二醛(MDA)及一氧化氮(NO)表达的影响。方法选取SPF级雄性SD大鼠30只,出生180d,体质量180～220g,高脂饲料喂养4周后再小剂量腹腔注射链脲佐菌素(STZ)诱导2型糖尿病模型,随机分为糖尿病对照组,GSPE250mg·kg-1·d-1、50mg·kg-1·d-1剂量干预组;另设6只为普食对照组。检测各组喂养第6周、10周后的血糖水平,并检测第10周后大鼠血清中SOD、MDA、GSH及NO的变化。结果GSPE能降低血糖,但效果不明显。在抗氧化指标的检测中,与糖尿病对照组相比,GSPE250mg·kg-1·d-1剂量干预组SOD、GSH明显升高(P<0.05),MDA、NO明显降低(P<0.05);50mg·kg-1·d-1剂量干预组SOD、GSH有所升高,MDA、NO有所降低,但差异无统计学意义(P>0.05)。结论较高剂量的GSPE能有效改善2型糖尿病大鼠的抗氧化能力,对其作用机制的进一步研究,可为GSPE对糖尿病及其并发症的营养干预治疗提供新的思路和依据。

Effects of proanthocyanidins,a grape-seed extract,on serum levels of SOD,MDA,GSH and NO in rats with type-2 diabetes

Objective To observe the effects of grape seed proanthocyanidins extract （GSPE） on serum levels of superoxide dismutase （SOD）, malondialdehyde （MDA）, reduced glutathione hormone （GSH） and nitric oxide （NO） in rats with type-2 diabetes. Methods Thirty specific pathogen-free SD rats （180 days from birth, 180-220 g in weight） were fed on high-fat chow for 4 weeks and then received intraperitoneal injection of small dose STZ to induce type-2 diabetes. These rats were randomized into the diabetic control group, 250 mg·kg^-1·d^-1 GPSE and 50 mg·kg^-1·d^-1 GSPE groups. Another 6 SD rats fed on usual diet were set as normal controls. In these rats, blood sugar was detected at weeks 6 and 10, and changes in SOD, MDA, GSH and NO at week 10. Results The level of blood sugar was decreased but not remarkably with GSPE. Compared with diabetic controls, remarkably increased levels of SOD and MDA and decreased levels of MDA and NO were found in the 250 mg·kg^-1·d^-1 GSPE group （all P〈0.05）. Similar results were seen in the 50 mg·kg^-1·d^-1 GSPE, but these were not statistically significant. Conclusion Sub-high dose of GSPE may be effective to improve the anti-oxidative capability in type-2 diabetic rats. Further study of the mechanism can provide new ideas and evidences for nutritional interventions with GSPE in diabetes and related complications.