涎腺腺样囊性癌中PCNA、P16和P27的表达及其临床意义

目的 :研究 PCNA、P1 6、P2 7基因在涎腺腺样囊性癌中的表达及其临床意义。方法 :采用 SP免疫组织化学染色方法检测 5 5例涎腺腺样囊性癌中 PCNA和 P1 6、P2 7基因蛋白的表达 ,使用 SPSS统计软件进行统计分析。结果 :PCNA在所有病例中均有不同程度的表达 ,其表达程度在组织类型、肿瘤部位和肿瘤分期方面存在着显著差异性 (P<0 .0 5 ) ,而在局部复发、转移和累积生存率方面无显著性差异。 P2 7基因阳性表达率为 34.5 % ,其表达程度在肿瘤部位和肿瘤分期方面存在着显著性差异 (P <0 .0 5 ) ,而在组织类型、局部复发、转移和累积生存率方面无显著性差异。 P1 6 基因阳性表达率为 6 5 .5 % ,其表达程度在所有变量均无显著性差异。结论 :在涎腺腺样囊性癌中 ,PCNA和 P2 7基因作为评估该肿瘤预后的生物学指标需要进一步的研究分析

Expressions of proliferating cell nuclear antigen, P16and P27 in adenoid cystic carcinoma of salivary glands

Objective:To evaluate the clinical significance of expression of proliferation cell nuclear antigen(PCNA),P16 and P27genes in adenoid cystic carcinoma of salivary glands.Methods:The expressions of PCNA,P16and P27were detected by immunohistochemical staining (SP)in 55 patients with adenoid cystic carcinoma of salivary glands.Results:The higher and lower expressions of PCNA were 45.5%(19/55) and 65.5%(35/55) respectively;of P16 were 45.5%(25/55) and 54.5%(30/55)respectively.And the positive and negative expressions of P27 were 34.5%(19/55) and 65.5%(36/55),respectively.The correlation of expression of PCNA gene with local recurrence and tumor site showed significant (P=0.0317,P=0.0186);but the expression of P16was no any significant correlation in all of the variables.The expression of P27 was significant on the regional lymph node metastaisis (P=0.0083).When higher expression of P16was 45.5%(25/55),the P27positive expression was 56.0%(14/25),which showed a significant positive correlation (P=0.0025).Conclusion:In adenoid cystic carcinoma of salivary glands,the PCNA gene should be one of the biological target predicting local recurrence,and P27gene should be one of the biological target justifying regional lymph node metastasis.