高效液相色谱法同时测定多种食品添加剂方法研究

目的]建立高效液相色谱法同时测定食品中安赛蜜、苯甲酸、山梨酸、糖精钠。方法]水超声提取样品，必要时用亚铁氰化钾溶液（106g／L）和乙酸锌溶液（220g／L）净化；样液采用Kromasil C18柱（150mm×4．6mm，5μm）色谱柱，流动相为甲醇-0．02mol／L乙酸铵溶液（6：94，V／V），二极管阵列检测器进行检测；以保留时间和紫外吸收光谱图结合定性，外标法测定食品种4种食品添加剂含量。结果]4种食品添加剂的检出限分别为安赛蜜9．00×10^-5mg／ml、苯甲酸1．20×10^-4mg／ml、山梨酸1．00×10^-4mg／ml、糖精钠1．60×10^-4mg／ml；各组分回收率在89．0％～100．0％之间；各组分测定RSD均小于5％。结论]该法特异性强、灵敏度高，操作简便快速，测定结果可靠，适用于食品中上述4种添加剂的常规分析。

Simultaneous Determination of Various Food Additives by High Performance Liquid Chromatography

Objective]To establish a method for simultaneous determination of acesulfame potassium, benzoic acid, sorbic acid and saccharin in food by high performance liquid chromatography. Methods] Samples were extracted with water by ultrasonic cleaner and,if necessary, were purified with potassium ferrocyanide solution（106 g/L） and zinc acetate solution（220g/L）. Then sample solutions were performed on Kromasil C18 （150 mm × 4.6 mm, 5 μm） and detected by diode array detector, with the mobile phase consisted of methanol -0.02 mol/L ammonium acetate （6 ： 94, V/V）. Food additives mentioned above were identified on the basis of the retention times and UV absorption spectrogram, and quantitative determined by the external standard method. Resuits]LODs for acesulfame potassium, benzoic acid, sorbic acid and saccharin were 9.00 × 10^-5 mg/ml, 1.20 × 10^-4 mg/ml, 1.00 × 10^-4 mg/ml and 1.60 × 10^-4 mg/ml, respectively. Recoveries of them were all between 89.0%- 100.0%, and RSD were less than 5 %. Conclusion]The method was accuracy , sensitive and simple,proved to be suitable-for the routine analysis of food additives mentioned above in food.