| 枸杞多糖的精制工艺优选 |
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| 引用本文： | 王结鑫,褚海清,赵雨,潘琼,王渊学,张立明. 枸杞多糖的精制工艺优选[J]. 湖南中医杂志, 2021, 37(2): 150-152,158. DOI: 10.16808/j.cnki.issn1003-7705.2021.02.052 |
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| 作者姓名： | 王结鑫 褚海清 赵雨 潘琼 王渊学 张立明 |
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| 作者单位： | 宁夏医科大学药学院,宁夏银川,750004;宁夏医科大学药学院,宁夏银川,750004;宁夏特色中医药现代化工程技术研究中心,宁夏银川,750004;宁夏医科大学回医药现代化教育部重点实验室,宁夏银川,750004 |
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| 基金项目： | 国家自然科学基金项目(81760839);宁夏自治区重点研发项目(2018BEG03014)。 |
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| 摘 要： | 目的:优化枸杞多糖(LBP)的精制工艺,为LBP的开发提供参考.方法:以多糖提取率为指标,采用正交试验考察提取次数、提取时间、料液比对多糖提取工艺的影响.在单因素试验的基础上,以糖损失率为指标,利用正交试验优化活性炭除色素工艺,以蛋白清除率和糖损失率的综合评分为指标,采用正交试验优化酶-sevag法脱蛋白工艺.结果:最...
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| 关 键 词： | 枸杞多糖 提取工艺 除色素 脱蛋白 |
Optimization of the purification process for Lycium barbarum polysaccharides |
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| WANG Jiexin,CHU Haiqing,ZHAO Yu,PAN Qiong,WANG Yuanxue,ZHANG Liming. Optimization of the purification process for Lycium barbarum polysaccharides[J]. Hunan Journal of Traditional Chinese Medicine, 2021, 37(2): 150-152,158. DOI: 10.16808/j.cnki.issn1003-7705.2021.02.052 |
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| Authors: | WANG Jiexin CHU Haiqing ZHAO Yu PAN Qiong WANG Yuanxue ZHANG Liming |
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| Affiliation: | (School of Pharmacy,Ningxia Medical University,Yinchuan 750004,Ningxia,China;Ningxia Engineering and Technology Research Center for Modernization of Chinese Medicine,Yinchuan 750004,Ningxia,China;Key Laboratory of Hui Ethnic Medicine Modernization,Ministry of Education,Ningxia Medical University,Yinchuan 750004,Ningxia,China) |
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| Abstract: | Objective:To optimize the purification process for Lycium barbarum polysaccharides(LBP),and to provide a reference for the development of LBP.Methods:With the extraction rate of polysaccharides as an index,the orthogonal test was used to evaluate the influence of times of extraction,extraction time,and solid-liquid ratio on the extraction process for LBP.On the basis of single-factor experiment,with the loss rate of polysaccharides as an index,the orthogonal test was used to optimize the depigmentation process with activated carbon,and with the comprehensive score of protein clearance rate and polysaccharide loss rate as an index,the orthogonal test was used to optimize the deproteinization process based on the enzyme-Sevag method.Results:The optimal extraction process was extraction twice,an extraction time of 30 minutes,and a solid-liquid ratio of 1∶10.The optimal depigmentation process was an amount of activated carbon used of 1.0%,a temperature of 100℃,and a time of 20 minutes;the polysaccharide loss rate was 28.6%.The optimal deproteinization process was an amount of enzyme used of 1.5%,a temperature of 50℃,and a time of 60 minutes;the protein clearance rate was 28.2%and the polysaccharide loss rate was 10.9%.Conclusion:The optimized purification process for LBP is stable,feasible,and suitable for the development of LBP products. |
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| Keywords: | Lycium barbarum polysaccharides extraction process depigmentation deproteinization |
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